Porcine CS ELISA Kit

Alternative name
Chondroitin sulfate ELISA Kit,CS ELISA Kit
Catalog
E078781
species
Porcine
GeneCS
SpecificityNo cross-reaction with other factors
Sensitivity0.38 ng/mL
Intended UsePorcine CS ELISA Kit allows for the in vitro quantitative determination of CS , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole kit at 4℃
Product Description
specificalPorcine CS ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in th is kit has been pre-coated with an antibody specific to CS, During the reaction,CS in the sample or standard competes with a fixed amount of biotin-labeled CS for sites on a pre-coated Monoclonal antibody specific to CS. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Porcine Citrate synthase, mitochondrial Protein information
Uniprot ID CISY_PIG
Uniprot AC P00889;
UniGene Ssc.16315;
GeneID 397519
KEGG ssc:397519;
Porcine Citrate synthase, mitochondrial Protein SEQUENCE
SEQUENCE 464 AA; 51629 MW; 66719A7504DF322A CRC64;
MALLTAAARL FGAKNASCLV LAARHASASS TNLKDILADL IPKEQARIKT
FRQQHGNTVV GQITVDMMYG GMRGMKGLVY ETSVLDPDEG IRFRGYSIPE
CQKMLPKAKG GEEPLPEGLF WLLVTGQIPT EEQVSWLSKE WAKRAALPSH
VVTMLDNFPT NLHPMSQLSA AITALNSESN FARAYAEGIH RTKYWELIYE
DCMDLIAKLP CVAAKIYRNL YREGSSIGAI DSKLDWSHNF TNMLGYTDAQ
FTELMRLYLT IHSDHEGGNV SAHTSHLVGS ALSDPYLSFA AAMNGLAGPL
HGLANQEVLV WLTQLQKEVG KDVSDEKLRD YIWNTLNSGR VVPGYGHAVL
RKTDPRYTCQ REFALKHLPH DPMFKLVAQL YKIVPNVLLE QGKAKNPWPN
VDAHSGVLLQ YYGMTEMNYY TVLFGVSRAL GVLAQLIWSR ALGFPLERPK
SMSTDGLIKL VDSK

Category

Porcine CS ELISA Kit

Chondroitin sulfate ELISA Kit,CS ELISA Kit