Human CRBN ELISA Kit

Alternative name
Human MRT2 ELISA Kit;Human MRT2A ELISA Kit;Human cereblon ELISA Kit;Human protein cereblon ELISA Kit;Human protein x 0001 ELISA Kit;
Catalog
E007857
species
Human
GeneCRBN
Standard Curve
Other Species Mouse Crbn ELISA Kit Bovine CRBN ELISA Kit Chicken CRBN ELISA Kit Rat Crbn ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of CRBN. No significant cross-reactivity or interference between CRBN and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human CRBN is typically less than 0.125ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by addi
Intended UseHuman CRBN ELISA Kit allows for the in vitro quantitative determination of CRBN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CRBN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to CRBN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CRBN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CRBN in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Protein cereblon Protein information
Uniprot ID CRBN_HUMAN
Uniprot AC Q96SW2; B2R6H4; C9IZA9; C9JAH6; Q6AI62; Q6NVZ0; Q9UHW4;
UniGene Hs.18925;
GeneID 51185
KEGG hsa:51185;
Human Protein cereblon Protein SEQUENCE
SEQUENCE 442 AA; 50546 MW; 90DF77D98A8BEAA8 CRC64;
MAGEGDQQDA AHNMGNHLPL LPAESEEEDE MEVEDQDSKE AKKPNIINFD
TSLPTSHTYL GADMEEFHGR TLHDDDSCQV IPVLPQVMMI LIPGQTLPLQ
LFHPQEVSMV RNLIQKDRTF AVLAYSNVQE REAQFGTTAE IYAYREEQDF
GIEIVKVKAI GRQRFKVLEL RTQSDGIQQA KVQILPECVL PSTMSAVQLE
SLNKCQIFPS KPVSREDQCS YKWWQKYQKR KFHCANLTSW PRWLYSLYDA
ETLMDRIKKQ LREWDENLKD DSLPSNPIDF SYRVAACLPI DDVLRIQLLK
IGSAIQRLRC ELDIMNKCTS LCCKQCQETE ITTKNEIFSL SLCGPMAAYV
NPHGYVHETL TVYKACNLNL IGRPSTEHSW FPGYAWTVAQ CKICASHIGW
KFTATKKDMS PQKFWGLTRS ALLPTIPDTE DEISPDKVIL CL
UCSC uc003bpq.4; human. [Q96SW2-1];

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Category

Human CRBN ELISA Kit

Human MRT2 ELISA Kit;Human MRT2A ELISA Kit;Human cereblon ELISA Kit;Human protein cereblon ELISA Kit;Human protein x 0001 ELISA Kit;