Human CERKL ELISA Kit

Alternative name
Human RP26 ELISA Kit;Human ceramide kinase like ELISA Kit;Human ceramide kinase-like protein ELISA Kit;
Catalog
E007839
species
Human
GeneCERKL
Standard Curve
Other Species Mouse CERKL ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of CERKL. No significant cross-reactivity or interference between CERKL and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CERKL and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseHuman CERKL ELISA Kit allows for the in vitro quantitative determination of CERKL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalIntended Uses: This CERKL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CERKL. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||CERKL ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-CERKL antibody and an CERKL-HRP conjugate. The assay sample and buffer are incubated together with CERKL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CERKL concentration since CERKL from samples and CERKL-HRP conjugate compete for the anti-CERKL antibody binding site. Since the number of sites is limited, as more sites are occupied by CERKL from the sample, fewer sites are left to bind CERKL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CERKL concentration in each sample is interpolated from this standard curve.
Human Ceramide kinase-like protein Protein information
Uniprot ID CERKL_HUMAN
Uniprot AC Q5DVJ4; Q5DVJ5; Q6UZF6; Q6ZP59;
UniGene Hs.732358;
GeneID 375298
KEGG hsa:375298;
Human Ceramide kinase-like protein Protein SEQUENCE
SEQUENCE 558 AA; 62622 MW; 305B6BB82CE977CB CRC64;
MPWRRRRNRV SALEGGREEE APPEAAAVPP ALLTSPQQTE AAAERILLRG
IFEIGRDSCD VVLSERALRW RPIQPERPAG DSKYDLLCKE EFIELKDIFS
VKLKRRCSVK QQRSGTLLGI TLFICLKKEQ NKLKNSTLDL INLSEDHCDI
WFRQFKKILA GFPNRPKSLK ILLNPQSHKK EATQVYYEKV EPLLKLAGIK
TDVTIMEYEG HALSLLKECE LQGFDGGHRK PLFAIHWSVQ RLFTGMQTLE
PSVVCVGGDG SASEVAHALL LRAQKNAGME TDRILTPVRA QLPLGLIPAG
STNVLAHSLH GVPHVITATL HIIMGHVQLV DVCTFSTAGK LLRFGFSAMF
GFGGRTLALA EKYRWMSPNQ RRDFAVVKAL AKLKAEDCEI SFLPFNSSDD
VQERRAQGSP KSDCNDQWQM IQGQFLNVSI MAIPCLCSVA PRGLAPNTRL
NNGSMALIIA RNTSRPEFIK HLKRYASVKN QFNFPFVETY TVEEVKVHPR
NNTGGYNPEE EEDETASENC FPWNVDGDLM EVASEVHIRL HPRLISLYGG
SMEEMIPK
UCSC uc002unx.4; human. [Q49MI3-1];

Category

Human CERKL ELISA Kit

Human RP26 ELISA Kit;Human ceramide kinase like ELISA Kit;Human ceramide kinase-like protein ELISA Kit;