Human UGCG ELISA Kit

Alternative name
Human GLCT-1 ELISA Kit;Human glucosylceramide synthase ELISA Kit;Human GCS ELISA Kit;Human UDP-glucose ceramide glucosyltransferase ELISA Kit;Human UDP-glucose:N-acylsphingosine D-glucosyltransferase ELISA Kit;Human GLCT1 ELISA Kit;Human ceramide glucosyltransferase ELISA Kit;
Catalog
E007833
species
Human
GeneUGCG
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.469ng/ml
Intended UseHuman UGCG ELISA Kit allows for the in vitro quantitative determination of UGCG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-UGCG antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-UGCG antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the UGCG amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of UGCG can be calculated.
Human Ceramide glucosyltransferase Protein information
Uniprot ID CEGT_HUMAN
Uniprot AC Q16739; Q5T258;
UniGene Hs.304249; Hs.593014;
GeneID 7357
KEGG hsa:7357;
Human Ceramide glucosyltransferase Protein SEQUENCE
SEQUENCE 394 AA; 44854 MW; 3B998569F8A96449 CRC64;
MALLDLALEG MAVFGFVLFL VLWLMHFMAI IYTRLHLNKK ATDKQPYSKL
PGVSLLKPLK GVDPNLINNL ETFFELDYPK YEVLLCVQDH DDPAIDVCKK
LLGKYPNVDA RLFIGGKKVG INPKINNLMP GYEVAKYDLI WICDSGIRVI
PDTLTDMVNQ MTEKVGLVHG LPYVADRQGF AATLEQVYFG TSHPRYYISA
NVTGFKCVTG MSCLMRKDVL DQAGGLIAFA QYIAEDYFMA KAIADRGWRF
AMSTQVAMQN SGSYSISQFQ SRMIRWTKLR INMLPATIIC EPISECFVAS
LIIGWAAHHV FRWDIMVFFM CHCLAWFIFD YIQLRGVQGG TLCFSKLDYA
VAWFIRESMT IYIFLSALWD PTISWRTGRY RLRCGGTAEE ILDV
UCSC uc004bft.4; human.;

Category

Human UGCG ELISA Kit

Human GLCT-1 ELISA Kit;Human glucosylceramide synthase ELISA Kit;Human GCS ELISA Kit;Human UDP-glucose ceramide glucosyltransferase ELISA Kit;Human UDP-glucose:N-acylsphingosine D-glucosyltransferase ELISA Kit;Human GLCT1 ELISA Kit;Human ceramide glucosyltransferase ELISA Kit;