Human UGCG ELISA Kit

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  • Alternative name

    Human GLCT-1 ELISA Kit;Human glucosylceramide synthase ELISA Kit;Human GCS ELISA Kit;Human UDP-glucose ceramide glucosyltransferase ELISA Kit;Human UDP-glucose:N-acylsphingosine D-glucosyltransferase ELISA Kit;Human GLCT1 ELISA Kit;Human ceramide glucosyltransferase ELISA Kit;

  • Catalog
    E007833
  • species
    Human
  • GeneUGCG
  • Other Species Mouse Ugcg ELISA KitRat Ugcg ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.469ng/ml
  • Intended UseHuman UGCG ELISA Kit allows for the in vitro quantitative determination of UGCG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-UGCG antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-UGCG antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the UGCG amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of UGCG can be calculated.
  • Human Ceramide glucosyltransferase Protein information
  • Uniprot ID CEGT_HUMAN
  • Uniprot AC Q16739; Q5T258;
  • UniGene Hs.304249; Hs.593014;
  • GeneID 7357
  • KEGG hsa:7357;
  • Human Ceramide glucosyltransferase Protein SEQUENCE
  • SEQUENCE 394 AA; 44854 MW; 3B998569F8A96449 CRC64;

    MALLDLALEG MAVFGFVLFL VLWLMHFMAI IYTRLHLNKK ATDKQPYSKL

    PGVSLLKPLK GVDPNLINNL ETFFELDYPK YEVLLCVQDH DDPAIDVCKK

    LLGKYPNVDA RLFIGGKKVG INPKINNLMP GYEVAKYDLI WICDSGIRVI

    PDTLTDMVNQ MTEKVGLVHG LPYVADRQGF AATLEQVYFG TSHPRYYISA

    NVTGFKCVTG MSCLMRKDVL DQAGGLIAFA QYIAEDYFMA KAIADRGWRF

    AMSTQVAMQN SGSYSISQFQ SRMIRWTKLR INMLPATIIC EPISECFVAS

    LIIGWAAHHV FRWDIMVFFM CHCLAWFIFD YIQLRGVQGG TLCFSKLDYA

    VAWFIRESMT IYIFLSALWD PTISWRTGRY RLRCGGTAEE ILDV

  • UCSC uc004bft.4; human.;


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