Human CENPF ELISA Kit

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  • Alternative name

    Human CENP-F ELISA Kit;Human AH antigen ELISA Kit;Human kinetochore protein CENPF ELISA Kit;Human mitosin ELISA Kit;Human CENF ELISA Kit;Human CILD31 ELISA Kit;Human PRO1779 ELISA Kit;Human STROMS ELISA Kit;Human hcp-1 ELISA Kit;Human centromere protein F ELISA Kit;Human CENP-F kinetochore protein ELISA Kit;Human cell-cycle-dependent 350K nuclear protein ELISA Kit;Human centromere protein F, 350/400kDa ELISA Kit;

  • Catalog
    E007775
  • species
    Human
  • GeneCENPF
  • Standard CurveHuman CENPF ELISA Kit
  • Other Species Mouse CENPF ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Centromere Protein F (CENPF). No significant cross-reactivity or interference between Centromere Protein F (CENPF) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.056ng/mL.
  • Intended UseHuman CENPF ELISA Kit allows for the in vitro quantitative determination of CENPF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Centromere Protein F (CENPF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Centromere Protein F (CENPF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Centromere Protein F (CENPF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Centromere Protein F (CENPF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Centromere protein F Protein information
  • Uniprot ID CENPF_HUMAN
  • Uniprot AC P49454; Q13171; Q13246; Q5VVM7;
  • UniGene Hs.497741;
  • GeneID 1063
  • KEGG hsa:1063;
  • Human Centromere protein F Protein SEQUENCE
  • SEQUENCE 3210 AA; 367764 MW; FF20F99216257BAD CRC64;

    MSWALEEWKE GLPTRALQKI QELEGQLDKL KKEKQQRQFQ LDSLEAALQK

    QKQKVENEKT EGTNLKRENQ RLMEICESLE KTKQKISHEL QVKESQVNFQ

    EGQLNSGKKQ IEKLEQELKR CKSELERSQQ AAQSADVSLN PCNTPQKIFT

    TPLTPSQYYS GSKYEDLKEK YNKEVEERKR LEAEVKALQA KKASQTLPQA

    TMNHRDIARH QASSSVFSWQ QEKTPSHLSS NSQRTPIRRD FSASYFSGEQ

    EVTPSRSTLQ IGKRDANSSF FDNSSSPHLL DQLKAQNQEL RNKINELELR

    LQGHEKEMKG QVNKFQELQL QLEKAKVELI EKEKVLNKCR DELVRTTAQY

    DQASTKYTAL EQKLKKLTED LSCQRQNAES ARCSLEQKIK EKEKEFQEEL

    SRQQRSFQTL DQECIQMKAR LTQELQQAKN MHNVLQAELD KLTSVKQQLE

    NNLEEFKQKL CRAEQAFQAS QIKENELRRS MEEMKKENNL LKSHSEQKAR

    EVCHLEAELK NIKQCLNQSQ NFAEEMKAKN TSQETMLRDL QEKINQQENS

    LTLEKLKLAV ADLEKQRDCS QDLLKKREHH IEQLNDKLSK TEKESKALLS

    ALELKKKEYE ELKEEKTLFS CWKSENEKLL TQMESEKENL QSKINHLETC

    LKTQQIKSHE YNERVRTLEM DRENLSVEIR NLHNVLDSKS VEVETQKLAY

    MELQQKAEFS DQKHQKEIEN MCLKTSQLTG QVEDLEHKLQ LLSNEIMDKD

    RCYQDLHAEY ESLRDLLKSK DASLVTNEDH QRSLLAFDQQ PAMHHSFANI

    IGEQGSMPSE RSECRLEADQ SPKNSAILQN RVDSLEFSLE SQKQMNSDLQ

    KQCEELVQIK GEIEENLMKA EQMHQSFVAE TSQRISKLQE DTSAHQNVVA

    ETLSALENKE KELQLLNDKV ETEQAEIQEL KKSNHLLEDS LKELQLLSET

    LSLEKKEMSS IISLNKREIE ELTQENGTLK EINASLNQEK MNLIQKSESF

    ANYIDEREKS ISELSDQYKQ EKLILLQRCE ETGNAYEDLS QKYKAAQEKN

    SKLECLLNEC TSLCENRKNE LEQLKEAFAK EHQEFLTKLA FAEERNQNLM

    LELETVQQAL RSEMTDNQNN SKSEAGGLKQ EIMTLKEEQN KMQKEVNDLL

    QENEQLMKVM KTKHECQNLE SEPIRNSVKE RESERNQCNF KPQMDLEVKE

    ISLDSYNAQL VQLEAMLRNK ELKLQESEKE KECLQHELQT IRGDLETSNL

    QDMQSQEISG LKDCEIDAEE KYISGPHELS TSQNDNAHLQ CSLQTTMNKL

    NELEKICEIL QAEKYELVTE LNDSRSECIT ATRKMAEEVG KLLNEVKILN

    DDSGLLHGEL VEDIPGGEFG EQPNEQHPVS LAPLDESNSY EHLTLSDKEV

    QMHFAELQEK FLSLQSEHKI LHDQHCQMSS KMSELQTYVD SLKAENLVLS

    TNLRNFQGDL VKEMQLGLEE GLVPSLSSSC VPDSSSLSSL GDSSFYRALL

    EQTGDMSLLS NLEGAVSANQ CSVDEVFCSS LQTYVDSLKA ENLVLSTNLR

    NFQGDLVKEM QLGLEEGLVP SLSSSCVPDS SSLSSLGDSS FYRALLEQTG

    DMSLLSNLEG VVSANQCSVD EVFCSSLQEE NLTRKETPSA PAKGVEELES

    LCEVYRQSLE KLEEKMESQG IMKNKEIQEL EQLLSSERQE LDCLRKQYLS

    ENEQWQQKLT SVTLEMESKL AAEKKQTEQL SLELEVARLQ LQGLDLSSRS

    LLGIDTEDAI QGRNESCDIS KEHTSETTER TPKHDVHQIC DKDAQQDLNL

    DIEKITETGA VKPTGECSGE QSPDTNYEPP GEDKTQGSSE CISELSFSGP

    NALVPMDFLG NQEDIHNLQL RVKETSNENL RLLHVIEDRD RKVESLLNEM

    KELDSKLHLQ EVQLMTKIEA CIELEKIVGE LKKENSDLSE KLEYFSCDHQ

    ELLQRVETSE GLNSDLEMHA DKSSREDIGD NVAKVNDSWK ERFLDVENEL

    SRIRSEKASI EHEALYLEAD LEVVQTEKLC LEKDNENKQK VIVCLEEELS

    VVTSERNQLR GELDTMSKKT TALDQLSEKM KEKTQELESH QSECLHCIQV

    AEAEVKEKTE LLQTLSSDVS ELLKDKTHLQ EKLQSLEKDS QALSLTKCEL

    ENQIAQLNKE KELLVKESES LQARLSESDY EKLNVSKALE AALVEKGEFA

    LRLSSTQEEV HQLRRGIEKL RVRIEADEKK QLHIAEKLKE RERENDSLKD

    KVENLERELQ MSEENQELVI LDAENSKAEV ETLKTQIEEM ARSLKVFELD

    LVTLRSEKEN LTKQIQEKQG QLSELDKLLS SFKSLLEEKE QAEIQIKEES

    KTAVEMLQNQ LKELNEAVAA LCGDQEIMKA TEQSLDPPIE EEHQLRNSIE

    KLRARLEADE KKQLCVLQQL KESEHHADLL KGRVENLERE LEIARTNQEH

    AALEAENSKG EVETLKAKIE GMTQSLRGLE LDVVTIRSEK ENLTNELQKE

    QERISELEII NSSFENILQE KEQEKVQMKE KSSTAMEMLQ TQLKELNERV

    AALHNDQEAC KAKEQNLSSQ VECLELEKAQ LLQGLDEAKN NYIVLQSSVN

    GLIQEVEDGK QKLEKKDEEI SRLKNQIQDQ EQLVSKLSQV EGEHQLWKEQ

    NLELRNLTVE LEQKIQVLQS KNASLQDTLE VLQSSYKNLE NELELTKMDK

    MSFVEKVNKM TAKETELQRE MHEMAQKTAE LQEELSGEKN RLAGELQLLL

    EEIKSSKDQL KELTLENSEL KKSLDCMHKD QVEKEGKVRE EIAEYQLRLH

    EAEKKHQALL LDTNKQYEVE IQTYREKLTS KEECLSSQKL EIDLLKSSKE

    ELNNSLKATT QILEELKKTK MDNLKYVNQL KKENERAQGK MKLLIKSCKQ

    LEEEKEILQK ELSQLQAAQE KQKTGTVMDT KVDELTTEIK ELKETLEEKT

    KEADEYLDKY CSLLISHEKL EKAKEMLETQ VAHLCSQQSK QDSRGSPLLG

    PVVPGPSPIP SVTEKRLSSG QNKASGKRQR SSGIWENGRG PTPATPESFS

    KKSKKAVMSG IHPAEDTEGT EFEPEGLPEV VKKGFADIPT GKTSPYILRR

    TTMATRTSPR LAAQKLALSP LSLGKENLAE SSKPTAGGSR SQKVKVAQRS

    PVDSGTILRE PTTKSVPVNN LPERSPTDSP REGLRVKRGR LVPSPKAGLE

    SNGSENCKVQ

  • UCSC uc001hkm.4; human.;


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