Human centromere autoantigen A ELISA Kit;Human centromere protein A ELISA Kit;Human CENP-A ELISA Kit;Human CenH3 ELISA Kit;Human histone H3-like centromeric protein A ELISA Kit;Human centromere protein A, 17kDa ELISA Kit;Human centromere-specific histone ELISA Kit;
SpecificityThis assay has high sensitivity and excellent specificity for detection of CENPA. No significant cross-reactivity or interference between CENPA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CENPA and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseHuman CENPA ELISA Kit allows for the in vitro quantitative determination of CENPA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyEpigenetics and Nuclear Signaling
Product Description specificalPrinciple of the assay: CENPA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CENPA antibody and an CENPA-HRP conjugate. The assay sample and buffer are incubated together with CENPA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CENPA concentration since CENPA from samples and CENPA-HRP conjugate compete for the anti-CENPA antibody binding site. Since the number of sites is limited, as more sites are occupied by CENPA from the sample, fewer sites are left to bind CENPA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CENPA concentration in each sample is interpolated from this standard curve.
Human Histone H3-like centromeric protein A Protein information
P49450; D6W544; Q53T74; Q9BVW2;
Human Histone H3-like centromeric protein A Protein SEQUENCE