Human CENPA ELISA Kit

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  • Alternative name

    Human centromere autoantigen A ELISA Kit;Human centromere protein A ELISA Kit;Human CENP-A ELISA Kit;Human CenH3 ELISA Kit;Human histone H3-like centromeric protein A ELISA Kit;Human centromere protein A, 17kDa ELISA Kit;Human centromere-specific histone ELISA Kit;

  • Catalog
    E007765
  • species
    Human
  • GeneCENPA
  • Standard CurveHuman CENPA ELISA Kit
  • Other Species Mouse Cenpa ELISA KitBovine CENPA ELISA KitChicken CENPA ELISA KitHamster CENPA ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CENPA. No significant cross-reactivity or interference between CENPA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CENPA and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman CENPA ELISA Kit allows for the in vitro quantitative determination of CENPA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyEpigenetics and Nuclear Signaling
  • Product Description
    specifical
    Principle of the assay: CENPA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CENPA antibody and an CENPA-HRP conjugate. The assay sample and buffer are incubated together with CENPA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CENPA concentration since CENPA from samples and CENPA-HRP conjugate compete for the anti-CENPA antibody binding site. Since the number of sites is limited, as more sites are occupied by CENPA from the sample, fewer sites are left to bind CENPA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CENPA concentration in each sample is interpolated from this standard curve.
  • Human Histone H3-like centromeric protein A Protein information
  • Uniprot ID CENPA_HUMAN
  • Uniprot AC P49450; D6W544; Q53T74; Q9BVW2;
  • UniGene Hs.1594;
  • GeneID 1058
  • KEGG hsa:1058;
  • Human Histone H3-like centromeric protein A Protein SEQUENCE
  • SEQUENCE 140 AA; 15991 MW; 11A28FEB54486489 CRC64;

    MGPRRRSRKP EAPRRRSPSP TPTPGPSRRG PSLGASSHQH SRRRQGWLKE

    IRKLQKSTHL LIRKLPFSRL AREICVKFTR GVDFNWQAQA LLALQEAAEA

    FLVHLFEDAY LLTLHAGRVT LFPKDVQLAR RIRGLEEGLG

  • UCSC uc002rhr.4; human. [P49450-1];




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