Human ADORA2B ELISA Kit

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  • Alternative name

    Human ADORA2 ELISA Kit;Human adenosine A2b receptor ELISA Kit;Human adenosine receptor A2b ELISA Kit;

  • Catalog
    E000776
  • species
    Human
  • GeneADORA2B
  • Standard CurveHuman ADORA2B ELISA Kit
  • Other Species Mouse Adora2b ELISA KitBovine ADORA2B ELISA KitCanine ADORA2B ELISA KitChicken ADORA2B ELISA KitRabbit ADORA2B ELISA KitRat Adora2b ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Adenosine A2b Receptor (ADORA2b). No significant cross-reactivity or interference between Adenosine A2b Receptor (ADORA2b) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.061ng/mL.
  • Intended UseHuman ADORA2B ELISA Kit allows for the in vitro quantitative determination of ADORA2B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenosine A2b Receptor (ADORA2b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adenosine A2b Receptor (ADORA2b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenosine A2b Receptor (ADORA2b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adenosine A2b Receptor (ADORA2b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Adenosine receptor A2b Protein information
  • Uniprot ID AA2BR_HUMAN
  • Uniprot AC P29275;
  • UniGene Hs.167046;
  • GeneID 136
  • KEGG hsa:136;
  • Human Adenosine receptor A2b Protein SEQUENCE
  • SEQUENCE 332 AA; 36333 MW; 38AD088E2439C7FA CRC64;

    MLLETQDALY VALELVIAAL SVAGNVLVCA AVGTANTLQT PTNYFLVSLA

    AADVAVGLFA IPFAITISLG FCTDFYGCLF LACFVLVLTQ SSIFSLLAVA

    VDRYLAICVP LRYKSLVTGT RARGVIAVLW VLAFGIGLTP FLGWNSKDSA

    TNNCTEPWDG TTNESCCLVK CLFENVVPMS YMVYFNFFGC VLPPLLIMLV

    IYIKIFLVAC RQLQRTELMD HSRTTLQREI HAAKSLAMIV GIFALCWLPV

    HAVNCVTLFQ PAQGKNKPKW AMNMAILLSH ANSVVNPIVY AYRNRDFRYT

    FHKIISRYLL CQADVKSGNG QAGVQPALGV GL

  • UCSC uc002gpd.2; human.;




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