Human CRABP1 ELISA Kit

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  • Alternative name

    Human cellular retinoic acid-binding protein I ELISA Kit;Human CRABP-I ELISA Kit;Human RBP5 ELISA Kit;Human CRABP ELISA Kit;Human CRABPI ELISA Kit;Human cellular retinoic acid binding protein 1 ELISA Kit;Human cellular retinoic acid-binding protein 1 ELISA Kit;

  • Catalog
    E007719
  • species
    Human
  • GeneCRABP1
  • Standard CurveHuman CRABP1 ELISA Kit
  • Other Species Bovine CRABP1 ELISA KitChicken CRABP1 ELISA KitMouse Crabp1 ELISA KitRat Crabp1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CRABP1. No significant cross-reactivity or interference between CRABP1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CRABP1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman CRABP1 ELISA Kit allows for the in vitro quantitative determination of CRABP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical
    Priciple of the assay: CRABP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CRABP1 antibody and an CRABP1-HRP conjugate. The assay sample and buffer are incubated together with CRABP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CRABP1 concentration since CRABP1 from samples and CRABP1-HRP conjugate compete for the anti-CRABP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CRABP1 from the sample, fewer sites are left to bind CRABP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CRABP1 concentration in each sample is interpolated from this standard curve.
  • Human Cellular retinoic acid-binding protein 1 Protein information
  • Uniprot ID RABP1_HUMAN
  • Uniprot AC P29762; Q6IAY7; Q8WTV5;
  • UniGene Hs.346950;
  • GeneID 1381
  • KEGG hsa:1381;
  • Human Cellular retinoic acid-binding protein 1 Protein SEQUENCE
  • SEQUENCE 137 AA; 15566 MW; A3DB048973E4E8C4 CRC64;

    MPNFAGTWKM RSSENFDELL KALGVNAMLR KVAVAAASKP HVEIRQDGDQ

    FYIKTSTTVR TTEINFKVGE GFEEETVDGR KCRSLATWEN ENKIHCTQTL

    LEGDGPKTYW TRELANDELI LTFGADDVVC TRIYVRE

  • UCSC uc002bdp.2; human.;


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