Human CD200R1 ELISA Kit

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  • Alternative name

    Human CD200 cell surface glycoprotein receptor ELISA Kit;Human cell surface glycoprotein OX2 receptor 1 ELISA Kit;Human CD200R ELISA Kit;Human CRTR2 ELISA Kit;Human MOX2R ELISA Kit;Human OX2R ELISA Kit;Human HCRTR2 ELISA Kit;Human CD200 receptor 1 ELISA Kit;Human cell surface glycoprotein CD200 receptor 1 ELISA Kit;Human MOX2 receptor ELISA Kit;Human cell surface glycoprotein receptor CD200 ELISA Kit;

  • Catalog
    E007707
  • species
    Human
  • GeneCD200R1
  • Standard CurveHuman CD200R1 ELISA Kit
  • Other Species Mouse Cd200r1 ELISA KitBovine CD200R1 ELISA KitRat Cd200r1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CD200R1. No significant cross-reactivity or interference between CD200R1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CD200R1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman CD200R1 ELISA Kit allows for the in vitro quantitative determination of CD200R1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical
    Principle of the assay: CD200R1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CD200R1 antibody and an CD200R1-HRP conjugate. The assay sample and buffer are incubated together with CD200R1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD200R1 concentration since CD200R1 from samples and CD200R1-HRP conjugate compete for the anti-CD200R1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CD200R1 from the sample, fewer sites are left to bind CD200R1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD200R1 concentration in each sample is interpolated from this standard curve.
  • Human Cell surface glycoprotein CD200 receptor 1 Protein information
  • Uniprot ID MO2R1_HUMAN
  • Uniprot AC Q8TD45; Q8TD52;
  • UniGene Hs.309158;
  • GeneID 131450
  • KEGG hsa:131450;
  • Human Cell surface glycoprotein CD200 receptor 1 Protein SEQUENCE
  • SEQUENCE 325 AA; 36620 MW; DD3F4B1F62337BBD CRC64;

    MLCPWRTANL GLLLILTIFL VAASSSLCMD EKQITQNYSK VLAEVNTSWP

    VKMATNAVLC CPPIALRNLI IITWEIILRG QPSCTKAYRK ETNETKETNC

    TDERITWVSR PDQNSDLQIR PVAITHDGYY RCIMVTPDGN FHRGYHLQVL

    VTPEVTLFQN RNRTAVCKAV AGKPAAQISW IPEGDCATKQ EYWSNGTVTV

    KSTCHWEVHN VSTVTCHVSH LTGNKSLYIE LLPVPGAKKS AKLYIPYIIL

    TIIILTIVGF IWLLKVNGCR KYKLNKTEST PVVEEDEMQP YASYTEKNNP

    LYDTTNKVKA SEALQSEVDT DLHTL

  • UCSC uc003dzj.2; human. [Q8TD46-1];




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