Human GPA33 ELISA Kit

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  • Alternative name

    Human glycoprotein A33 ELISA Kit;Human A33 ELISA Kit;Human cell surface A33 antigen ELISA Kit;Human glycoprotein A33 (transmembrane) ELISA Kit;Human transmembrane glycoprotein A33 ELISA Kit;

  • Catalog
    E007705
  • species
    Human
  • GeneGPA33
  • Other Species Mouse Gpa33 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.375ng/ml
  • Intended UseHuman GPA33 ELISA Kit allows for the in vitro quantitative determination of GPA33 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human GPA33 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human GPA33 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human GPA33 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human GPA33 can be calculated.
  • Human Cell surface A33 antigen Protein information
  • Uniprot ID GPA33_HUMAN
  • Uniprot AC Q99795; Q5VZP6;
  • UniGene Hs.651244;
  • GeneID 10223
  • KEGG hsa:10223;
  • Human Cell surface A33 antigen Protein SEQUENCE
  • SEQUENCE 319 AA; 35632 MW; 9BFC7AAF45C2408E CRC64;

    MVGKMWPVLW TLCAVRVTVD AISVETPQDV LRASQGKSVT LPCTYHTSTS

    SREGLIQWDK LLLTHTERVV IWPFSNKNYI HGELYKNRVS ISNNAEQSDA

    SITIDQLTMA DNGTYECSVS LMSDLEGNTK SRVRLLVLVP PSKPECGIEG

    ETIIGNNIQL TCQSKEGSPT PQYSWKRYNI LNQEQPLAQP ASGQPVSLKN

    ISTDTSGYYI CTSSNEEGTQ FCNITVAVRS PSMNVALYVG IAVGVVAALI

    IIGIIIYCCC CRGKDDNTED KEDARPNREA YEEPPEQLRE LSREREEEDD

    YRQEEQRSTG RESPDHLDQ

  • UCSC uc001gea.2; human.;



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