Human GPA33 ELISA Kit

Alternative name
Human glycoprotein A33 ELISA Kit;Human A33 ELISA Kit;Human cell surface A33 antigen ELISA Kit;Human glycoprotein A33 (transmembrane) ELISA Kit;Human transmembrane glycoprotein A33 ELISA Kit;
Catalog
E007705
species
Human
GeneGPA33
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.375ng/ml
Intended UseHuman GPA33 ELISA Kit allows for the in vitro quantitative determination of GPA33 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human GPA33 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human GPA33 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human GPA33 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human GPA33 can be calculated.
Human Cell surface A33 antigen Protein information
Uniprot ID GPA33_HUMAN
Uniprot AC Q99795; Q5VZP6;
UniGene Hs.651244;
GeneID 10223
KEGG hsa:10223;
Human Cell surface A33 antigen Protein SEQUENCE
SEQUENCE 319 AA; 35632 MW; 9BFC7AAF45C2408E CRC64;
MVGKMWPVLW TLCAVRVTVD AISVETPQDV LRASQGKSVT LPCTYHTSTS
SREGLIQWDK LLLTHTERVV IWPFSNKNYI HGELYKNRVS ISNNAEQSDA
SITIDQLTMA DNGTYECSVS LMSDLEGNTK SRVRLLVLVP PSKPECGIEG
ETIIGNNIQL TCQSKEGSPT PQYSWKRYNI LNQEQPLAQP ASGQPVSLKN
ISTDTSGYYI CTSSNEEGTQ FCNITVAVRS PSMNVALYVG IAVGVVAALI
IIGIIIYCCC CRGKDDNTED KEDARPNREA YEEPPEQLRE LSREREEEDD
YRQEEQRSTG RESPDHLDQ
UCSC uc001gea.2; human.;

Category

Human GPA33 ELISA Kit

Human glycoprotein A33 ELISA Kit;Human A33 ELISA Kit;Human cell surface A33 antigen ELISA Kit;Human glycoprotein A33 (transmembrane) ELISA Kit;Human transmembrane glycoprotein A33 ELISA Kit;