Human CDC20 ELISA Kit

Alternative name
Human p55CDC ELISA Kit;Human CDC20A ELISA Kit;Human bA276H19.3 ELISA Kit;Human cell division cycle 20 ELISA Kit;Human cell division cycle protein 20 homolog ELISA Kit;Human CDC20 cell division cycle 20 homolog ELISA Kit;Human cell division cycle 20 homolog ELISA Kit;
Catalog
E007695
species
Human
GeneCDC20
Standard Curve
Other Species Mouse Cdc20 ELISA Kit Porcine CDC20 ELISA Kit Rat Cdc20 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of CDC20. No significant cross-reactivity or interference between CDC20 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CDC20 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseHuman CDC20 ELISA Kit allows for the in vitro quantitative determination of CDC20 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalPrinciple of the assay: CDC20 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CDC20 antibody and an CDC20-HRP conjugate. The assay sample and buffer are incubated together with CDC20-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CDC20 concentration since CDC20 from samples and CDC20-HRP conjugate compete for the anti-CDC20 antibody binding site. Since the number of sites is limited, as more sites are occupied by CDC20 from the sample, fewer sites are left to bind CDC20-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CDC20 concentration in each sample is interpolated from this standard curve.
Human Cell division cycle protein 20 homolog Protein information
Uniprot ID CDC20_HUMAN
Uniprot AC Q12834; B2R6Z6; D3DPJ1; Q5JUY4; Q9BW56; Q9UQI9;
UniGene Hs.524947;
GeneID 991
KEGG hsa:991;
Human Cell division cycle protein 20 homolog Protein SEQUENCE
SEQUENCE 499 AA; 54723 MW; FD5C967AF84089E8 CRC64;
MAQFAFESDL HSLLQLDAPI PNAPPARWQR KAKEAAGPAP SPMRAANRSH
SAGRTPGRTP GKSSSKVQTT PSKPGGDRYI PHRSAAQMEV ASFLLSKENQ
PENSQTPTKK EHQKAWALNL NGFDVEEAKI LRLSGKPQNA PEGYQNRLKV
LYSQKATPGS SRKTCRYIPS LPDRILDAPE IRNDYYLNLV DWSSGNVLAV
ALDNSVYLWS ASSGDILQLL QMEQPGEYIS SVAWIKEGNY LAVGTSSAEV
QLWDVQQQKR LRNMTSHSAR VGSLSWNSYI LSSGSRSGHI HHHDVRVAEH
HVATLSGHSQ EVCGLRWAPD GRHLASGGND NLVNVWPSAP GEGGWVPLQT
FTQHQGAVKA VAWCPWQSNV LATGGGTSDR HIRIWNVCSG ACLSAVDAHS
QVCSILWSPH YKELISGHGF AQNQLVIWKY PTMAKVAELK GHTSRVLSLT
MSPDGATVAS AAADETLRLW RCFELDPARR REREKASAAK SSLIHQGIR
UCSC uc001cix.4; human.;

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Category

Human CDC20 ELISA Kit

Human p55CDC ELISA Kit;Human CDC20A ELISA Kit;Human bA276H19.3 ELISA Kit;Human cell division cycle 20 ELISA Kit;Human cell division cycle protein 20 homolog ELISA Kit;Human CDC20 cell division cycle 20 homolog ELISA Kit;Human cell division cycle 20 homolog ELISA Kit;