SpecificityThis assay has high sensitivity and excellent specificity for detection of CDKN2AIPNL. No significant cross-reactivity or interference between CDKN2AIPNL and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CDKN2AIPNL and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseHuman CDKN2AIPNL ELISA Kit allows for the in vitro quantitative determination of CDKN2AIPNL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description specificalIntended Uses: This CDKN2AIPNL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CDKN2AIPNL. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay||CDKN2AIPNL ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CDKN2AIPNL antibody and an CDKN2AIPNL-HRP conjugate. The assay sample and buffer are incubated together with CDKN2AIPNL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CDKN2AIPNL concentration since CDKN2AIPNL from samples and CDKN2AIPNL-HRP conjugate compete for the anti-CDKN2AIPNL antibody binding site. Since the number of sites is limited, as more sites are occupied by CDKN2AIPNL from the sample, fewer sites are left to bind CDKN2AIPNL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CDKN2AIPNL concentration in each sample is interpolated from this standard curve.
Human CDKN2AIP N-terminal-like protein Protein information
Human CDKN2AIP N-terminal-like protein Protein SEQUENCE