Human CDC42EP4 ELISA Kit

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  • Alternative name

    Human binder of Rho GTPases 4 ELISA Kit;Human BORG4 ELISA Kit;Human CEP4 ELISA Kit;Human KAIA1777 ELISA Kit;Human cdc42 effector protein 4 ELISA Kit;Human CDC42 effector protein (Rho GTPase binding) 4 ELISA Kit;

  • Catalog
    E007575
  • species
    Human
  • GeneCDC42EP4
  • Standard CurveHuman CDC42EP4 ELISA Kit
  • Other Species Mouse Cdc42ep4 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CDC42EP4. No significant cross-reactivity or interference between CDC42EP4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CDC42EP4 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman CDC42EP4 ELISA Kit allows for the in vitro quantitative determination of CDC42EP4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Intended Uses: This CDC42EP4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CDC42EP4. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||CDC42EP4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CDC42EP4 antibody and an CDC42EP4-HRP conjugate. The assay sample and buffer are incubated together with CDC42EP4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CDC42EP4 concentration since CDC42EP4 from samples and CDC42EP4-HRP conjugate compete for the anti-CDC42EP4 antibody binding site. Since the number of sites is limited, as more sites are occupied by CDC42EP4 from the sample, fewer sites are left to bind CDC42EP4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CDC42EP4 concentration in each sample is interpolated from this standard curve.
  • Human Cdc42 effector protein 4 Protein information
  • Uniprot ID BORG4_HUMAN
  • Uniprot AC Q9H3Q1; B3KUS7; O95828; Q96FT3;
  • UniGene Hs.3903;
  • GeneID 23580
  • KEGG hsa:23580;
  • Human Cdc42 effector protein 4 Protein SEQUENCE
  • SEQUENCE 356 AA; 37980 MW; 2CF677C60C6EF1B5 CRC64;

    MPILKQLVSS SVHSKRRSRA DLTAEMISAP LGDFRHTMHV GRAGDAFGDT

    SFLNSKAGEP DGESLDEQPS SSSSKRSLLS RKFRGSKRSQ SVTRGEREQR

    DMLGSLRDSA LFVKNAMSLP QLNEKEAAEK GTSKLPKSLS SSPVKKANDG

    EGGDEEAGTE EAVPRRNGAA GPHSPDPLLD EQAFGDLTDL PVVPKATYGL

    KHAESIMSFH IDLGPSMLGD VLSIMDKEEW DPEEGEGGYH GDEGAAGTIT

    QAPPYAVAAP PLARQEGKAG PDLPSLPSHA LEDEGWAAAA PSPGSARSMG

    SHTTRDSSSL SSCTSGILEE RSPAFRGPDR ARAAVSRQPD KEFSFMDEEE

    EDEIRV

  • UCSC uc002jjo.4; human. [Q9H3Q1-1];


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