Human CD99 ELISA Kit

Alternative name
Human 12E7 ELISA Kit;Human E2 antigen ELISA Kit;Human Protein MIC2 ELISA Kit;Human T-cell surface glycoprotein E2 ELISA Kit;Human MIC2 ELISA Kit;Human MIC2X ELISA Kit;Human MIC2Y ELISA Kit;Human HBA71 ELISA Kit;Human MSK5X ELISA Kit;Human CD99 molecule ELISA Kit;Human CD99 antigen ELISA Kit;Human MIC2 (monoclonal antibody 12E7) ELISA Kit;Human antigen identified by monoclonal 12E7, Y homolog ELISA Kit;Human antigen identified by monoclonal antibodies 12E7, F21 and O13 ELISA Kit;Human cell surface antigen 12E7 ELISA Kit;Human cell surface antigen HBA-71 ELISA Kit;Human cell surface antigen O13 ELISA Kit;Human surface antigen MIC2 ELISA Kit;
Catalog
E007557
species
Human
GeneCD99
Other Species Mouse Cd99 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human CD99. No significant cross-reactivity or interference between Human CD99 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human CD99 and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.094ng/mL
Intended UseHuman CD99 ELISA Kit allows for the in vitro quantitative determination of CD99 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CD99 in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CD99. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human CD99 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CD99, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human CD99. You can calculate the concentration of Human CD99 in the samples by comparing the OD of the samples to the standard curve.
Human CD99 antigen Protein information
Uniprot ID CD99_HUMAN
Uniprot AC P14209; A6NIW1; O00518; Q6ICV7;
UniGene Hs.653349;
GeneID 4267
KEGG hsa:4267;
Human CD99 antigen Protein SEQUENCE
SEQUENCE 185 AA; 18848 MW; C302E09E6B022EAB CRC64;
MARGAALALL LFGLLGVLVA APDGGFDLSD ALPDNENKKP TAIPKKPSAG
DDFDLGDAVV DGENDDPRPP NPPKPMPNPN PNHPSSSGSF SDADLADGVS
GGEGKGGSDG GGSHRKEGEE ADAPGVIPGI VGAVVVAVAG AISSFIAYQK
KKLCFKENAE QGEVDMESHR NANAEPAVQR TLLEK
UCSC uc004cqm.4; human. [P14209-1];

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Human CD99 ELISA Kit