Human CD97 ELISA Kit

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  • Alternative name

    Human leukocyte antigen CD97 ELISA Kit;Human CD97 ELISA Kit;Human TM7LN1 ELISA Kit;Human adhesion G protein-coupled receptor E5 ELISA Kit;Human CD97 antigen ELISA Kit;Human CD97 molecule ELISA Kit;Human seven transmembrane helix receptor ELISA Kit;Human seven-span transmembrane protein ELISA Kit;Human seven-transmembrane, heterodimeric receptor associated with inflammation ELISA Kit;

  • Catalog
    E007554
  • species
    Human
  • GeneCD97
  • Standard CurveHuman CD97 ELISA Kit
  • Other Species Mouse Cd97 ELISA KitBovine CD97 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman CD97 ELISA Kit allows for the in vitro quantitative determination of CD97 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyImmunology
  • Product Description
    specifical
    Principle of the Assay: CD97 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CD97 antibody and an CD97-HRP conjugate. The assay sample and buffer are incubated together with CD97-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD97 concentration since CD97 from samples and CD97-HRP conjugate compete for the anti-CD97 antibody binding site. Since the number of sites is limited, as more sites are occupied by CD97 from the sample, fewer sites are left to bind CD97-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD97 concentration in each sample is interpolated from this standard curve.
  • Human CD97 antigen subunit beta Protein information
  • Uniprot ID CD97_HUMAN
  • Uniprot AC P48960; A8K7Z4; B2RBJ9; O00718; O76101; Q8NG72; Q8TBQ7;
  • UniGene Hs.466039; Hs.531619;
  • GeneID 976
  • KEGG hsa:976;
  • Human CD97 antigen subunit beta Protein SEQUENCE
  • SEQUENCE 835 AA; 91869 MW; 06DDDE9178BC494B CRC64;

    MGGRVFLAFC VWLTLPGAET QDSRGCARWC PQNSSCVNAT ACRCNPGFSS

    FSEIITTPTE TCDDINECAT PSKVSCGKFS DCWNTEGSYD CVCSPGYEPV

    SGAKTFKNES ENTCQDVDEC QQNPRLCKSY GTCVNTLGSY TCQCLPGFKF

    IPEDPKVCTD VNECTSGQNP CHSSTHCLNN VGSYQCRCRP GWQPIPGSPN

    GPNNTVCEDV DECSSGQHQC DSSTVCFNTV GSYSCRCRPG WKPRHGIPNN

    QKDTVCEDMT FSTWTPPPGV HSQTLSRFFD KVQDLGRDSK TSSAEVTIQN

    VIKLVDELME APGDVEALAP PVRHLIATQL LSNLEDIMRI LAKSLPKGPF

    TYISPSNTEL TLMIQERGDK NVTMGQSSAR MKLNWAVAAG AEDPGPAVAG

    ILSIQNMTTL LANASLNLHS KKQAELEEIY ESSIRGVQLR RLSAVNSIFL

    SHNNTKELNS PILFAFSHLE SSDGEAGRDP PAKDVMPGPR QELLCAFWKS

    DSDRGGHWAT EGCQVLGSKN GSTTCQCSHL SSFAILMAHY DVEDWKLTLI

    TRVGLALSLF CLLLCILTFL LVRPIQGSRT TIHLHLCICL FVGSTIFLAG

    IENEGGQVGL RCRLVAGLLH YCFLAAFCWM SLEGLELYFL VVRVFQGQGL

    STRWLCLIGY GVPLLIVGVS AAIYSKGYGR PRYCWLDFEQ GFLWSFLGPV

    TFIILCNAVI FVTTVWKLTQ KFSEINPDMK KLKKARALTI TAIAQLFLLG

    CTWVFGLFIF DDRSLVLTYV FTILNCLQGA FLYLLHCLLN KKVREEYRKW

    ACLVAGGSKY SEFTSTTSGT GHNQTRALRA SESGI

  • UCSC uc002myl.4; human. [P48960-1];


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