Human APRT ELISA Kit

Alternative name
Human APRT ELISA Kit;Human AMP ELISA Kit;Human APRTD ELISA Kit;Human adenine phosphoribosyltransferase ELISA Kit;Human AMP diphosphorylase ELISA Kit;Human AMP pyrophosphorylase ELISA Kit;Human transphosphoribosidase ELISA Kit;
Catalog
E000753
species
Human
GeneAPRT
Standard Curve
Other Species Mouse Aprt ELISA Kit Bovine APRT ELISA Kit Hamster APRT ELISA Kit Rat Aprt ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of APRT. No significant cross-reactivity or interference between APRT and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of APRT is typically less than 0.057ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two
Intended UseHuman APRT ELISA Kit allows for the in vitro quantitative determination of APRT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to APRT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to APRT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain APRT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of APRT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Adenine phosphoribosyltransferase Protein information
Uniprot ID APT_HUMAN
Uniprot AC P07741; G5E9J2; Q3KP55; Q68DF9;
UniGene Hs.28914;
GeneID 353
KEGG hsa:353;
Human Adenine phosphoribosyltransferase Protein SEQUENCE
SEQUENCE 180 AA; 19608 MW; CDC7703337A6E453 CRC64;
MADSELQLVE QRIRSFPDFP TPGVVFRDIS PVLKDPASFR AAIGLLARHL
KATHGGRIDY IAGLDSRGFL FGPSLAQELG LGCVLIRKRG KLPGPTLWAS
YSLEYGKAEL EIQKDALEPG QRVVVVDDLL ATGGTMNAAC ELLGRLQAEV
LECVSLVELT SLKGREKLAP VPFFSLLQYE
UCSC uc002flv.4; human. [P07741-1];

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Category

Human APRT ELISA Kit

Human APRT ELISA Kit;Human AMP ELISA Kit;Human APRTD ELISA Kit;Human adenine phosphoribosyltransferase ELISA Kit;Human AMP diphosphorylase ELISA Kit;Human AMP pyrophosphorylase ELISA Kit;Human transphosphoribosidase ELISA Kit;