Human APRT ELISA Kit

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  • Alternative name

    Human APRT ELISA Kit;Human AMP ELISA Kit;Human APRTD ELISA Kit;Human adenine phosphoribosyltransferase ELISA Kit;Human AMP diphosphorylase ELISA Kit;Human AMP pyrophosphorylase ELISA Kit;Human transphosphoribosidase ELISA Kit;

  • Catalog
    E000753
  • species
    Human
  • GeneAPRT
  • Standard CurveHuman APRT ELISA Kit
  • Other Species Mouse Aprt ELISA KitBovine APRT ELISA KitHamster APRT ELISA KitRat Aprt ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of APRT. No significant cross-reactivity or interference between APRT and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of APRT is typically less than 0.057ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two
  • Intended UseHuman APRT ELISA Kit allows for the in vitro quantitative determination of APRT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to APRT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to APRT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain APRT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of APRT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Adenine phosphoribosyltransferase Protein information
  • Uniprot ID APT_HUMAN
  • Uniprot AC P07741; G5E9J2; Q3KP55; Q68DF9;
  • UniGene Hs.28914;
  • GeneID 353
  • KEGG hsa:353;
  • Human Adenine phosphoribosyltransferase Protein SEQUENCE
  • SEQUENCE 180 AA; 19608 MW; CDC7703337A6E453 CRC64;

    MADSELQLVE QRIRSFPDFP TPGVVFRDIS PVLKDPASFR AAIGLLARHL

    KATHGGRIDY IAGLDSRGFL FGPSLAQELG LGCVLIRKRG KLPGPTLWAS

    YSLEYGKAEL EIQKDALEPG QRVVVVDDLL ATGGTMNAAC ELLGRLQAEV

    LECVSLVELT SLKGREKLAP VPFFSLLQYE

  • UCSC uc002flv.4; human. [P07741-1];


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