Human CD63 ELISA Kit

Alternative name
Human granulophysin ELISA Kit;Human lysosomal-associated membrane protein 3 ELISA Kit;Human LAMP-3 ELISA Kit;Human melanoma-associated antigen ME491 ELISA Kit;Human OMA81H ELISA Kit;Human ocular melanoma-associated antigen ELISA Kit;Human tetraspanin-30 ELISA Kit;Human tspan-30 ELISA Kit;Human MLA1 ELISA Kit;Human TSPAN30 ELISA Kit;Human ME491 ELISA Kit;Human CD63 molecule ELISA Kit;Human CD63 antigen ELISA Kit;Human CD63 antigen (melanoma 1 antigen) ELISA Kit;Human lysosome-associated membrane glycoprotein 3 ELISA Kit;Human melanoma-associated antigen MLA1 ELISA Kit;
Catalog
E007514
species
Human
GeneCD63
SpecificityThis assay has high sensitivity and excellent specificity for detection of human CD63. No significant cross-reactivity or interference between human CD63 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human CD63 is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
Intended UseHuman CD63 ELISA Kit allows for the in vitro quantitative determination of CD63 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CD63 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD63 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD63 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD63 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human CD63 antigen Protein information
Uniprot ID CD63_HUMAN
Uniprot AC P08962; F8VZE2; Q5TZP3; Q8N6Z9; Q9UCG6;
UniGene Hs.445570;
GeneID 967
KEGG hsa:967;
Human CD63 antigen Protein SEQUENCE
SEQUENCE 238 AA; 25637 MW; 85AC8E235C6E425F CRC64;
MAVEGGMKCV KFLLYVLLLA FCACAVGLIA VGVGAQLVLS QTIIQGATPG
SLLPVVIIAV GVFLFLVAFV GCCGACKENY CLMITFAIFL SLIMLVEVAA
AIAGYVFRDK VMSEFNNNFR QQMENYPKNN HTASILDRMQ ADFKCCGAAN
YTDWEKIPSM SKNRVPDSCC INVTVGCGIN FNEKAIHKEG CVEKIGGWLR
KNVLVVAAAA LGIAFVEVLG IVFACCLVKS IRSGYEVM
UCSC uc001shn.5; human. [P08962-1];

Category

Human CD63 ELISA Kit