Human ICAM1 ELISA Kit

Alternative name
Human ICAM-1 ELISA Kit;Human major group rhinovirus receptor ELISA Kit;Human BB2 ELISA Kit;Human CD54 ELISA Kit;Human P3.58 ELISA Kit;Human intercellular adhesion molecule 1 ELISA Kit;Human cell surface glycoprotein P3.58 ELISA Kit;Human intercellular adhesion molecule 1 (CD54), human rhinovirus receptor ELISA Kit;
Catalog
E007489
species
Human
GeneICAM1
Other Species Mouse Icam1 ELISA Kit Bovine ICAM1 ELISA Kit Canine ICAM1 ELISA Kit Rat Icam1 ELISA Kit
SpecificityThe Human CD54 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human CD54 proteins within the range of 62.5 pg/ml - 4000 pg/ml.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity30 pg/ml.
Intended UseHuman ICAM1 ELISA Kit allows for the in vitro quantitative determination of ICAM1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||The Human CD54 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD54 in Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates. This assay employs an antibody specific for Human CD54 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD54 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD54 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD54 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: Intercellular adhesion molecule-1 (ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated (LFA) antigens, a beta 2 leukocyte integrin. The normal function of human intercellular adhesion molecule-1 (ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen (LFA-1) or macrophage-1 antigen (Mac-1). ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines. ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses.
Human Intercellular adhesion molecule 1 Protein information
Uniprot ID ICAM1_HUMAN
Uniprot AC P05362; B2R6M3; Q5NKV7; Q96B50;
UniGene Hs.643447;
GeneID 3383
KEGG hsa:3383;
Human Intercellular adhesion molecule 1 Protein SEQUENCE
SEQUENCE 532 AA; 57825 MW; 550089365A733AFB CRC64;
MAPSSPRPAL PALLVLLGAL FPGPGNAQTS VSPSKVILPR GGSVLVTCST
SCDQPKLLGI ETPLPKKELL LPGNNRKVYE LSNVQEDSQP MCYSNCPDGQ
STAKTFLTVY WTPERVELAP LPSWQPVGKN LTLRCQVEGG APRANLTVVL
LRGEKELKRE PAVGEPAEVT TTVLVRRDHH GANFSCRTEL DLRPQGLELF
ENTSAPYQLQ TFVLPATPPQ LVSPRVLEVD TQGTVVCSLD GLFPVSEAQV
HLALGDQRLN PTVTYGNDSF SAKASVSVTA EDEGTQRLTC AVILGNQSQE
TLQTVTIYSF PAPNVILTKP EVSEGTEVTV KCEAHPRAKV TLNGVPAQPL
GPRAQLLLKA TPEDNGRSFS CSATLEVAGQ LIHKNQTREL RVLYGPRLDE
RDCPGNWTWP ENSQQTPMCQ AWGNPLPELK CLKDGTFPLP IGESVTVTRD
LEGTYLCRAR STQGEVTRKV TVNVLSPRYE IVIITVVAAA VIMGTAGLST
YLYNRQRKIK KYRLQQAQKG TPMKPNTQAT PP
UCSC uc002mnq.3; human.;

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Human ICAM1 ELISA Kit