Human CD47 ELISA Kit

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  • Alternative name

    Human antigenic surface determinant protein OA3 ELISA Kit;Human Integrin-associated protein ELISA Kit;Human IAP ELISA Kit;Human Protein MER6 ELISA Kit;Human MER6 ELISA Kit;Human OA3 ELISA Kit;Human CD47 molecule ELISA Kit;Human leukocyte surface antigen CD47 ELISA Kit;Human CD47 antigen (Rh-related antigen, integrin-associated signal transducer) ELISA Kit;Human CD47 glycoprotein ELISA Kit;Human Rh-related antigen ELISA Kit;Human antigen identified by monoclonal antibody 1D8 ELISA Kit;Human integrin associated protein ELISA Kit;Human integrin-associated signal transducer ELISA Kit;

  • Catalog
    E007471
  • species
    Human
  • GeneCD47
  • Standard CurveHuman CD47 ELISA Kit
  • Other Species Mouse Cd47 ELISA KitBovine CD47 ELISA KitPorcine CD47 ELISA KitRat Cd47 ELISA Kit
  • SpecificityNatural and recombinant human CD47
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity Sample For quantitative detection of human CD47 in cell culture supernates and serum.
  • Intended UseHuman CD47 ELISA Kit allows for the in vitro quantitative determination of CD47 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: Human CD47 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD47 has been precoated onto 96-well plates. Standards (NSO, Q19 - P139) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD47 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD47 amount of sample captured in plate. Background: CD47 (Cluster of Differentiation 47), also known as integrin associated protein (IAP), is a transmembrane protein that in humans is encoded by the CD47 gene. It belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPalpha). CD47 is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. Furthermore, it plays a key role in immune and angiogenic responses. Also CD47 is ubiquitously expressed in human cells and has found to be overexpressed in many different tumor cells.
  • Human Leukocyte surface antigen CD47 Protein information
  • Uniprot ID CD47_HUMAN
  • Uniprot AC Q08722; A8K198; D3DN59; Q53Y71; Q96A60;
  • UniGene Hs.446414;
  • GeneID 961
  • KEGG hsa:961;
  • Human Leukocyte surface antigen CD47 Protein SEQUENCE
  • SEQUENCE 323 AA; 35214 MW; 5D20730A2632D550 CRC64;

    MWPLVAALLL GSACCGSAQL LFNKTKSVEF TFCNDTVVIP CFVTNMEAQN

    TTEVYVKWKF KGRDIYTFDG ALNKSTVPTD FSSAKIEVSQ LLKGDASLKM

    DKSDAVSHTG NYTCEVTELT REGETIIELK YRVVSWFSPN ENILIVIFPI

    FAILLFWGQF GIKTLKYRSG GMDEKTIALL VAGLVITVIV IVGAILFVPG

    EYSLKNATGL GLIVTSTGIL ILLHYYVFST AIGLTSFVIA ILVIQVIAYI

    LAVVGLSLCI AACIPMHGPL LISGLSILAL AQLLGLVYMK FVASNQKTIQ

    PPRKAVEEPL NAFKESKGMM NDE

  • UCSC uc003dwt.2; human. [Q08722-1];


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