Human CD40L/TNFSF5 ELISA Kit

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  • Alternative name

    CD40 ligand ELISA KIT; T-cell antigen Gp39 ELISA KIT; TNF-related activation protein ELISA KIT; TRAP ELISA KIT; Tumor necrosis factor ligand superfamily member 5 ELISA KIT; CD_antigen: CD154Cleaved into the following 2 chains:CD40 ligand, membrane form ELISA KIT; CD40 ligand, soluble formCD40LG ELISA KIT; CD40L ELISA KIT; TNFSF5 ELISA KIT; TRAP ELISA KIT; CD40-L ELISA KIT; TRAP ELISA KIT

  • Catalog
    E007458
  • species
    Human
  • GeneCD40L/TNFSF5
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human CD40L/TNFSF5. No significant cross-reactivity or interference between Human CD40L/TNFSF5 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human CD40L/TNFSF5 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity37.5pg/mL
  • Intended UseHuman CD40L/TNFSF5 ELISA Kit allows for the in vitro quantitative determination of CD40L/TNFSF5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CD40L/TNFSF5 in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CD40L/TNFSF5. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human CD40L/TNFSF5 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CD40L/TNFSF5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human CD40L/TNFSF5. You can calculate the concentration of Human CD40L/TNFSF5 in the samples by comparing the OD of the samples to the standard curve.


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