Human CD28 ELISA Kit

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  • Alternative name

    Human Tp44 ELISA Kit;Human CD28 molecule ELISA Kit;Human T-cell-specific surface glycoprotein CD28 ELISA Kit;Human CD28 antigen ELISA Kit;

  • Catalog
    E007407
  • species
    Human
  • GeneCD28
  • Other Species Mouse Cd28 ELISA KitBovine CD28 ELISA KitChicken CD28 ELISA KitRabbit CD28 ELISA KitRat Cd28 ELISA Kit
  • SpecificityNatural and recombinant Human CD28
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity Intra-assay Precision Intra-Assay Precision (Precision within an assay) Three samples of kn
  • Intended UseHuman CD28 ELISA Kit allows for the in vitro quantitative determination of CD28 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: Human CD28 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD28 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: N19-P152) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD28 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin- Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CD28 amount of sample captured in plate. Background/Introduction: CD28 (Cluster of Differentiation 28) is one of the proteins expressed on T cells that provide costimulatory signals required for T cell activation and survival. T cell stimulation through CD28 in addition to the T-cell receptor (TCR) can provide a potent signal for the production of various interleukins (IL-6 in particular). The CD28 gene is mapped to 2q33-q34. CD28 and CTLA-4, together with their ligands, B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily.
  • Human T-cell-specific surface glycoprotein CD28 Protein information
  • Uniprot ID CD28_HUMAN
  • Uniprot AC Q8WXJ2; Q9BYV0;
  • UniGene Hs.443123;
  • GeneID 940
  • KEGG hsa:940;
  • Human T-cell-specific surface glycoprotein CD28 Protein SEQUENCE
  • SEQUENCE 220 AA; 25066 MW; 1D9B6552A5878D0F CRC64;

    MLRLLLALNL FPSIQVTGNK ILVKQSPMLV AYDNAVNLSC KYSYNLFSRE

    FRASLHKGLD SAVEVCVVYG NYSQQLQVYS KTGFNCDGKL GNESVTFYLQ

    NLYVNQTDIY FCKIEVMYPP PYLDNEKSNG TIIHVKGKHL CPSPLFPGPS

    KPFWVLVVVG GVLACYSLLV TVAFIIFWVR SKRSRLLHSD YMNMTPRRPG

    PTRKHYQPYA PPRDFAAYRS

  • UCSC uc002vah.6; human. [P10747-1];




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