Mouse Pfkfb2 ELISA Kit

Alternative name
Mouse 6PF-2-K/Fru-2,6-P2ase 2 ELISA Kit;Mouse PFK/FBPase 2 ELISA Kit;Mouse 6PF-2-K/Fru-2,6-P2ase heart-type isozyme ELISA Kit;Mouse 4930568D07Rik ELISA Kit;Mouse 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 ELISA Kit;Mouse 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 ELISA Kit;Mouse PFK-2/FBPase-2 gene B ELISA Kit;
Catalog
E073814
species
Mouse
GenePfkfb2
SpecificityThis assay has high sensitivity and excellent specificity for detection of PFKFB2. No significant cross-reactivity or interference between PFKFB2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PFKFB2 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseMouse Pfkfb2 ELISA Kit allows for the in vitro quantitative determination of Pfkfb2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPrinciple of the assay: PFKFB2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PFKFB2 antibody and an PFKFB2-HRP conjugate. The assay sample and buffer are incubated together with PFKFB2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PFKFB2 concentration since PFKFB2 from samples and PFKFB2-HRP conjugate compete for the anti-PFKFB2 antibody binding site. Since the number of sites is limited, as more sites are occupied by PFKFB2 from the sample, fewer sites are left to bind PFKFB2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PFKFB2 concentration in each sample is interpolated from this standard curve.
Mouse Fructose-2,6-bisphosphatase Protein information
Uniprot ID F262_MOUSE
Uniprot AC P70265; Q8VEI9;
UniGene Mm.249861;
GeneID 18640
KEGG mmu:18640;
Mouse Fructose-2,6-bisphosphatase Protein SEQUENCE
SEQUENCE 519 AA; 59925 MW; FD4CF41AEDF22F89 CRC64;
MSENSTFSTE DSCNSSYKPH ASNLRRAGKT CSWASYMTNS PTLIVMIGLP
ARGKTYVSKK LTRYLNWIGV PTKVFNLGVY RREAVKSYQS YDFFRHDNEE
AMKIRKQCAL VALEDVKAYF TEESGQIAVF DATNTTRERR DMILNFAKQN
AFKVFFVESV CDDPDVIAAN ILEVKVSSPD YPERNRENVM EDFLKRIECY
KVTYQPLDPD NYDKDLSFIK VMNVGQRFLV NRVQDYIQSK IVYYLMNIHV
HPRTIYLCRH GESEFNLLGK IGGDSGLSVR GKQFAHALKK FLEEQEIQDL
KVWTSQLKRT IQTAESLGVT YEQWKILNEI DAGVCEEMTY SEIEQRYPEE
FALRDQEKYL YRYPGGESYQ DLVQRLEPVI MELERQGNIL VISHQAVMRC
LLAYFLDKGA DELPYLRCPL HIIFKLTPVA YGCKVETITL NVDAVDTHRD
KPTHNFPKSQ TPVRMRRNSF TPLSSSNTIR RPRNYSVGSR PLKPLSPLRA
LDMQEGADQP KTQVSIPVV

Category

Mouse Pfkfb2 ELISA Kit