Human IL2RA ELISA Kit

Alternative name
Human IL-2 receptor subunit alpha ELISA Kit;Human IL-2-RA ELISA Kit;Human IL-2R subunit alpha ELISA Kit;Human IL2-RA ELISA Kit;Human TAC antigen ELISA Kit;Human p55 ELISA Kit;Human CD25 ELISA Kit;Human IDDM10 ELISA Kit;Human IL2R ELISA Kit;Human IMD41 ELISA Kit;Human TCGFR ELISA Kit;Human interleukin 2 receptor subunit alpha ELISA Kit;Human interleukin-2 receptor subunit alpha ELISA Kit;Human interleukin 2 receptor, alpha ELISA Kit;
Catalog
E007379
species
Human
GeneIL2RA
SpecificityThe Human CD25 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human CD25 proteins within the range of 31.3 pg/ml - 2000 pg/ml.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity15 pg/ml.
Intended UseHuman IL2RA ELISA Kit allows for the in vitro quantitative determination of IL2RA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||The Human CD25 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD25 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human CD25 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD25 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD25 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD25 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. The IL2R gene was mapped to 10p15-p14. CD25 is expressed in most B-cell neoplasms, some acute nonlymphocytic leukemias, neuroblastomas, and tumor infiltrating lymphocytes. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.
Human Interleukin-2 receptor subunit alpha Protein information
Uniprot ID IL2RA_HUMAN
Uniprot AC P01589; Q5W007;
UniGene Hs.231367;
GeneID 3559
KEGG hsa:3559;
Human Interleukin-2 receptor subunit alpha Protein SEQUENCE
SEQUENCE 272 AA; 30819 MW; 83D907C8C81D2C0E CRC64;
MDSYLLMWGL LTFIMVPGCQ AELCDDDPPE IPHATFKAMA YKEGTMLNCE
CKRGFRRIKS GSLYMLCTGN SSHSSWDNQC QCTSSATRNT TKQVTPQPEE
QKERKTTEMQ SPMQPVDQAS LPGHCREPPP WENEATERIY HFVVGQMVYY
QCVQGYRALH RGPAESVCKM THGKTRWTQP QLICTGEMET SQFPGEEKPQ
ASPEGRPESE TSCLVTTTDF QIQTEMAATM ETSIFTTEYQ VAVAGCVFLL
ISVLLLSGLT WQRRQRKSRR TI
UCSC uc001iiz.3; human.;

Human IL2RA ELISA Kit

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