-
Catalog
E073779
-
species
Mouse
-
Gene5HT
-
SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse 5HT . No significant cross-reactivity or interference between mouse 5HT and analogues was observed.
-
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
-
SensitivityThe minimum detectable dose of mouse 5HT is typically less than 125 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest mouse 5HT concentration that could be differentiated from zero. It was determined the mea
-
Intended UseMouse 5HT ELISA Kit allows for the in vitro quantitative determination of 5HT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
-
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
-
Product Description
specificalPrinciple of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 5HT. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated 5HT. The competitive inhibition reaction is launched between with HRP-conjugated 5HT and 5HT in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of 5HT in the sample. The color development is stopped and the intensity of the color is measured.
-