Mouse Tyrp1 ELISA Kit

Alternative name
Mouse DHICA oxidase ELISA Kit;Mouse brown locus protein ELISA Kit;Mouse catalase B ELISA Kit;Mouse tyrosinase-related protein 1 ELISA Kit;Mouse TRP ELISA Kit;Mouse TRP-1 ELISA Kit;Mouse TRP1 ELISA Kit;Mouse Tyrp-1 ELISA Kit;Mouse Oca3 ELISA Kit;Mouse Tyrp ELISA Kit;Mouse b ELISA Kit;Mouse brown ELISA Kit;Mouse isa ELISA Kit;Mouse 5,6-dihydroxyindole-2-carboxylic acid oxidase ELISA Kit;Mouse iris stromal atrophy ELISA Kit;
Catalog
E073753
species
Mouse
GeneTyrp1
Standard Curve
Other Species Human TYRP1 ELISA Kit Bovine TYRP1 ELISA Kit Chicken TYRP1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of TYRP1. No significant cross-reactivity or interference between TYRP1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TYRP1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseMouse Tyrp1 ELISA Kit allows for the in vitro quantitative determination of Tyrp1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCancer
Product Description
specificalIntended Uses||This TYRP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse TYRP1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: TYRP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TYRP1 antibody and an TYRP1-HRP conjugate. The assay sample and buffer are incubated together with TYRP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TYRP1 concentration since TYRP1 from samples and TYRP1-HRP conjugate compete for the anti-TYRP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by TYRP1 from the sample, fewer sites are left to bind TYRP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TYRP1 concentration in each sample is interpolated from this standard curve.
Mouse 5,6-dihydroxyindole-2-carboxylic acid oxidase Protein information
Uniprot ID TYRP1_MOUSE
Uniprot AC NQKILTVR RNLLDLSPEE KSHFVRALDM AKRTTHPQFV IATRRLEDIL GPDGNTPQFE
UniGene Mm.30438;
GeneID 22178
KEGG mmu:22178;
Mouse 5,6-dihydroxyindole-2-carboxylic acid oxidase Protein SEQUENCE
SEQUENCE 537 AA; 60761 MW; 86570998AA9EB0BC CRC64;
MKSYNVLPLA YISLFLMLFY QVWAQFPREC ANIEALRRGV CCPDLLPSSG
PGTDPCGSSS GRGRCVAVIA DSRPHSRHYP HDGKDDREAW PLRFFNRTCQ
CNDNFSGHNC GTCRPGWRGA ACNQKILTVR RNLLDLSPEE KSHFVRALDM
AKRTTHPQFV IATRRLEDIL GPDGNTPQFE NISVYNYFVW THYYSVKKTF
LGTGQESFGD VDFSHEGPAF LTWHRYHLLQ LERDMQEMLQ EPSFSLPYWN
FATGKNVCDV CTDDLMGSRS NFDSTLISPN SVFSQWRVVC ESLEEYDTLG
TLCNSTEGGP IRRNPAGNVG RPAVQRLPEP QDVTQCLEVR VFDTPPFYSN
STDSFRNTVE GYSAPTGKYD PAVRSLHNLA HLFLNGTGGQ THLSPNDPIF
VLLHTFTDAV FDEWLRRYNA DISTFPLENA PIGHNRQYNM VPFWPPVTNT
EMFVTAPDNL GYAYEVQWPG QEFTVSEIIT IAVVAALLLV AAIFGVASCL
IRSRSTKNEA NQPLLTDHYQ RYAEDYEELP NPNHSMV
UCSC uc008tjq.3; mouse.;

Category

Mouse Tyrp1 ELISA Kit