Mouse Abat ELISA Kit

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  • Alternative name

    Mouse (S)-3-amino-2-methylpropionate transaminase ELISA Kit;Mouse GABA aminotransferase ELISA Kit;Mouse GABA-AT ELISA Kit;Mouse gamma-amino-N-butyrate transaminase ELISA Kit;Mouse GABA transaminase ELISA Kit;Mouse GABA-T ELISA Kit;Mouse L-AIBAT ELISA Kit;Mouse Gabat ELISA Kit;Mouse 9630038C02Rik ELISA Kit;Mouse AI255750 ELISA Kit;Mouse Gabaat ELISA Kit;Mouse Gm9851 ELISA Kit;Mouse I54 ELISA Kit;Mouse Laibat ELISA Kit;Mouse X61497 ELISA Kit;Mouse 4-aminobutyrate aminotransferase ELISA Kit;Mouse 4-aminobutyrate aminotransferase, mitochondrial ELISA Kit;Mouse 4-aminobutyrate transaminase ELISA Kit;Mouse GABA transferase ELISA Kit;Mouse beta-alanine oxoglutarate aminotransferase ELISA Kit;

  • Catalog
    E073700
  • species
    Mouse
  • GeneAbat
  • Standard CurveMouse Abat ELISA Kit
  • Other Species Human ABAT ELISA KitBovine ABAT ELISA KitPorcine ABAT ELISA KitRat Abat ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ABAT. No significant cross-reactivity or interference between ABAT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ABAT and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse Abat ELISA Kit allows for the in vitro quantitative determination of Abat , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: ABAT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ABAT antibody and an ABAT-HRP conjugate. The assay sample and buffer are incubated together with ABAT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ABAT concentration since ABAT from samples and ABAT-HRP conjugate compete for the anti-ABAT antibody binding site. Since the number of sites is limited, as more sites are occupied by ABAT from the sample, fewer sites are left to bind ABAT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ABAT concentration in each sample is interpolated from this standard curve.
  • Mouse 4-aminobutyrate aminotransferase, mitochondrial Protein information
  • Uniprot ID GABT_MOUSE
  • Uniprot AC P61922; Q8BZA3;
  • UniGene Mm.259315;
  • GeneID 268860
  • KEGG mmu:268860;
  • Mouse 4-aminobutyrate aminotransferase, mitochondrial Protein SEQUENCE
  • SEQUENCE 500 AA; 56452 MW; 85AA331AB48355F3 CRC64;

    MAFLLITRRL ACSSQKNLHL FIPGSRYISQ AAAKVDIEFD YDGPLMKTEV

    PGPRSKELMK QLNTIQNAEA VHFFCNYEES RGNYLVDVDG NRMLDLYSQI

    SSVPIGYNHP ALAKLVQQPQ NASTFINRPA LGILPPENFV DKLQESLMSV

    APRGMSQLIT MACGSCSNEN AFKTIFMWYR SKERGQRGFS KEELETCMVN

    QSPGCPDYSI LSFMGAFHGR TMGCLATTHS KAIHKIDIPS FDWPIAPFPR

    LKYPLEEFTT DNQQEEARCL EEVEDLIVKY RKKKRTVAGI IVEPIQSEGG

    DNHASDDFFR KLRDIARKHG CAFLVDEVQT GGGCTGKFWA HEHWGLDDPA

    DVMTFSKKMM TGGFFHKEEF RPSAPYRIFN TWLGDPSKNL LLAEVINIIK

    REDLLNNVAR VGKTLLTGLL DLQAQYPQFI SRVRGRGTFC SFDTPDEAIR

    NKLILIARNK GVVLGGCGDK SIRFRPTLVF RDHHAHLFLS IFSGILADFK

  • UCSC uc007ycp.2; mouse. [P61922-2];


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