Intended UseMouse 2, 3-DPG ELISA Kit allows for the in vitro quantitative determination of 2, 3-DPG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalFor samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
INTENDED USE This 2,3-DPG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse 2,3-DPG. This ELISA kit for research use only,not for therapeutic or diagnostic applications!
PRINCIPLE OF THE ASSAY 2,3-DPG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-2,3-DPG antibody and an 2,3-DPG-HRP conjugate. The assay sample and buffer are incubated together with 2,3-DPG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 2,3-DPG concentration since 2,3-DPG from samples and 2,3-DPG-HRP conjugate compete for the anti-2,3-DPG antibody binding site. Since the number of sites is limited, as more sites are occupied by 2,3-DPG from the sample, fewer sites are left to bind 2,3-DPG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 2,3-DPG concentration in each sample is interpolated from this standard curve.