Intended UseMouse Zg16 ELISA Kit allows for the in vitro quantitative determination of Zg16 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Zymogen granule protein 16. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Zymogen granule protein 16 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Zymogen granule protein 16, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Zymogen granule protein 16 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse Zymogen granule membrane protein 16 Protein information
Mouse Zymogen granule membrane protein 16 Protein SEQUENCE