Human CD160 ELISA Kit

Alternative name
Human natural killer cell receptor BY55 ELISA Kit;Human BY55 ELISA Kit;Human NK1 ELISA Kit;Human NK28 ELISA Kit;Human CD160 molecule ELISA Kit;Human CD160 antigen ELISA Kit;Human CD160 transmembrane isoform ELISA Kit;Human CD160-delta Ig ELISA Kit;Human natural killer cell receptor, immunoglobulin superfamily member ELISA Kit;
Catalog
E007309
species
Human
GeneCD160
SpecificityThis assay has high sensitivity and excellent specificity for detection of CD160. No significant cross-reactivity or interference between CD160 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CD160 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman CD160 ELISA Kit allows for the in vitro quantitative determination of CD160 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyImmunology
Product Description
specificalPrinciple of the assay: CD160 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CD160 antibody and an CD160-HRP conjugate. The assay sample and buffer are incubated together with CD160-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD160 concentration since CD160 from samples and CD160-HRP conjugate compete for the anti-CD160 antibody binding site. Since the number of sites is limited, as more sites are occupied by CD160 from the sample, fewer sites are left to bind CD160-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD160 concentration in each sample is interpolated from this standard curve.
Human CD160 antigen Protein information
Uniprot ID BY55_HUMAN
Uniprot AC O95971;
UniGene Hs.488237; Hs.740780;
GeneID 11126
KEGG hsa:11126;
Human CD160 antigen Protein SEQUENCE
SEQUENCE 181 AA; 19810 MW; EF0B981ACC7478BD CRC64;
MLLEPGRGCC ALAILLAIVD IQSGGCINIT SSASQEGTRL NLICTVWHKK
EEAEGFVVFL CKDRSGDCSP ETSLKQLRLK RDPGIDGVGE ISSQLMFTIS
QVTPLHSGTY QCCARSQKSG IRLQGHFFSI LFTETGNYTV TGLKQRQHLE
FSHNEGTLSS GFLQEKVWVM LVTSLVALQA L

Human CD160 ELISA Kit

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