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Human CD14  ELISA Kit

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  • Alternative name

    Monocyte differentiation antigen CD14 ELISA KIT; Myeloid cell-specific leucine-rich glycoprotein ELISA KIT; CD_antigen: CD14CD14 ELISA KIT;

  • Catalog
    E007304
  • species
    Human
  • GeneCD14 
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CD14. No significant cross-reactivity or interference between CD14 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CD14 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman CD14  ELISA Kit allows for the in vitro quantitative determination of CD14  , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyImmunology
  • Product Description
    specifical    Intended Uses: This CD14 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CD14. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: CD14 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CD14 antibody and an CD14-HRP conjugate. The assay sample and buffer are incubated together with CD14-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD14 concentration since CD14 from samples and CD14-HRP conjugate compete for the anti-CD14 antibody binding site. Since the number of sites is limited, as more sites are occupied by CD14 from the sample, fewer sites are left to bind CD14-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD14 concentration in each sample is interpolated from this standard curve.



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