Human CD14 ELISA Kit

Alternative name
Human myeloid cell-specific leucine-rich glycoprotein ELISA Kit;Human CD14 molecule ELISA Kit;Human monocyte differentiation antigen CD14 ELISA Kit;
Catalog
E007295
species
Human
GeneCD14
SpecificityThis assay has high sensitivity and excellent specificity for detection of human sCD14. No significant cross-reactivity or interference between human sCD14 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.229 ng/ml.
Intended UseHuman CD14 ELISA Kit allows for the in vitro quantitative determination of CD14 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for sCD14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sCD14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for sCD14 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sCD14 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Monocyte differentiation antigen CD14, membrane-bound form Protein information
Uniprot ID CD14_HUMAN
Uniprot AC P08571; Q53XT5; Q96FR6; Q96L99; Q9UNS3;
UniGene Hs.163867;
GeneID 929
KEGG hsa:929;
Human Monocyte differentiation antigen CD14, membrane-bound form Protein SEQUENCE
SEQUENCE 375 AA; 40076 MW; 1746CDB41F394F8D CRC64;
MERASCLLLL LLPLVHVSAT TPEPCELDDE DFRCVCNFSE PQPDWSEAFQ
CVSAVEVEIH AGGLNLEPFL KRVDADADPR QYADTVKALR VRRLTVGAAQ
VPAQLLVGAL RVLAYSRLKE LTLEDLKITG TMPPLPLEAT GLALSSLRLR
NVSWATGRSW LAELQQWLKP GLKVLSIAQA HSPAFSCEQV RAFPALTSLD
LSDNPGLGER GLMAALCPHK FPAIQNLALR NTGMETPTGV CAALAAAGVQ
PHSLDLSHNS LRATVNPSAP RCMWSSALNS LNLSFAGLEQ VPKGLPAKLR
VLDLSCNRLN RAPQPDELPE VDNLTLDGNP FLVPGTALPH EGSMNSGVVP
ACARSTLSVG VSGTLVLLQG ARGFA
UCSC uc003lgi.3; human.;

Category

Human CD14 ELISA Kit

Human myeloid cell-specific leucine-rich glycoprotein ELISA Kit;Human CD14 molecule ELISA Kit;Human monocyte differentiation antigen CD14 ELISA Kit;