-
Alternative name
DNA repair protein complementing XP-C cells ELISA KIT; Xeroderma pigmentosum group C-complementing protein ELISA KIT; p125XPC ELISA KIT; XPCC ELISA KIT
-
Catalog
E072907
-
species
Mouse
-
GeneXPC
-
SpecificityThis assay has high sensitivity and excellent specificity for detection of Xeroderma Pigmentosum, Complementation Group C (XPC).
No significant cross-reactivity or interference between Xeroderma Pigmentosum, Complementation Group C (XPC) and analogues was observed.
-
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
-
SensitivityTypically less than 0.055ng/mL.
-
Intended UseMouse XPC ELISA Kit allows for the in vitro quantitative determination of XPC , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
-
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
-
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Xeroderma Pigmentosum, Complementation Group C (XPC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Xeroderma Pigmentosum, Complementation Group C (XPC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Xeroderma Pigmentosum, Complementation Group C (XPC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Xeroderma Pigmentosum, Complementation Group C (XPC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
-