Human TNFRSF1A ELISA Kit

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  • Alternative name

    Human Tumor necrosis factor receptor 1 ELISA Kit;Human TNF-R1 ELISA Kit;Human Tumor necrosis factor receptor type I ELISA Kit;Human TNF-RI ELISA Kit;Human TNFR-I ELISA Kit;Human p55 ELISA Kit;Human p60 ELISA Kit;Human TBPI ELISA Kit;Human TNFAR ELISA Kit;Human TNFR1 ELISA Kit;Human CD120a ELISA Kit;Human FPF ELISA Kit;Human TBP1 ELISA Kit;Human TNF-R ELISA Kit;Human TNF-R-I ELISA Kit;Human TNF-R55 ELISA Kit;Human TNFR55 ELISA Kit;Human TNFR60 ELISA Kit;Human p55-R ELISA Kit;Human TNF receptor superfamily member 1A ELISA Kit;Human tumor necrosis factor receptor superfamily member 1A ELISA Kit;Human tumor necrosis factor binding protein 1 ELISA Kit;Human tumor necrosis factor receptor 1A isoform beta ELISA Kit;Human tumor necrosis factor receptor type 1 ELISA Kit;Human tumor necrosis factor-alpha receptor ELISA Kit;

  • Catalog
    E007279
  • species
    Human
  • GeneTNFRSF1A
  • Standard CurveHuman TNFRSF1A ELISA Kit
  • Other Species Mouse Tnfrsf1a ELISA KitBovine TNFRSF1A ELISA KitPorcine TNFRSF1A ELISA KitRat Tnfrsf1a ELISA Kit
  • SpecificityThe Human CD120a ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human CD120a proteins within the range of 7.8 pg/ml - 500 pg/ml.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of Human CD120a is typically less than 1 pg/ml.
  • Intended UseHuman TNFRSF1A ELISA Kit allows for the in vitro quantitative determination of TNFRSF1A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: The Human CD120a ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD120a in Cell Culture Supernatants, Serum, Plasma, Urine. This assay employs an antibody specific for Human CD120a coated on a 96-well plate. Standards and samples are pipetted into the wells and CD120a present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD120a antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD120a bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background: TNFsRI (also known as Solube tumor necrosis factor receptor 1) is a cytokine receptor that binds tumor necrosis factors (TNF). In contrast, the p60 (TNFRSF1A) and p80 (TNFRSF1B) TNFA receptors self-assemble through a distinct functional domain in the TNFR extracellular domain, termed the pre-ligand assembly domain (PLAD), in the absence of ligand. Deletion of the PLAD results in monomeric presentation of p60 or p80. Flow cytometric analysis showed that efficient TNFA binding depends on receptor self-assembly. They also found that other members of the TNF receptor superfamily, including the extracellular domains of TRAIL (TNFRSF10A), CD40, and FAS (TNFRSF6), all self-associate but do not interact with heterologous receptors. By Southern blot analysis of human/Chinese hamster somatic cell hybrid DNA, the TNFR1 gene was mapped to 12pter-cen. And by nonradioactive in situ hybridization that the type 1 receptor (the p55 TNF receptor) is encoded by a gene located on chromosome 12p13.2.
  • Human Tumor necrosis factor-binding protein 1 Protein information
  • Uniprot ID TNR1A_HUMAN
  • Uniprot AC Q9UCA4;
  • UniGene Hs.279594; Hs.713833;
  • GeneID 7132
  • KEGG hsa:7132;
  • Human Tumor necrosis factor-binding protein 1 Protein SEQUENCE
  • SEQUENCE 455 AA; 50495 MW; 4CEFBA96D03B8225 CRC64;

    MGLSTVPDLL LPLVLLELLV GIYPSGVIGL VPHLGDREKR DSVCPQGKYI

    HPQNNSICCT KCHKGTYLYN DCPGPGQDTD CRECESGSFT ASENHLRHCL

    SCSKCRKEMG QVEISSCTVD RDTVCGCRKN QYRHYWSENL FQCFNCSLCL

    NGTVHLSCQE KQNTVCTCHA GFFLRENECV SCSNCKKSLE CTKLCLPQIE

    NVKGTEDSGT TVLLPLVIFF GLCLLSLLFI GLMYRYQRWK SKLYSIVCGK

    STPEKEGELE GTTTKPLAPN PSFSPTPGFT PTLGFSPVPS STFTSSSTYT

    PGDCPNFAAP RREVAPPYQG ADPILATALA SDPIPNPLQK WEDSAHKPQS

    LDTDDPATLY AVVENVPPLR WKEFVRRLGL SDHEIDRLEL QNGRCLREAQ

    YSMLATWRRR TPRREATLEL LGRVLRDMDL LGCLEDIEEA LCGPAALPPA

    PSLLR

  • UCSC uc001qnu.4; human. [P19438-1];



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