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Mouse Wif1 ELISA Kit

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  • Alternative name

    Mouse WIF-1 ELISA Kit;Mouse AW107799 ELISA Kit;Mouse wnt inhibitory factor 1 ELISA Kit;
  • Catalog
    E072759
  • species
    Mouse
  • GeneWif1
  • SpecificityNatural and recombinant Mouse WIF1
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity Intra-assay Precision Intra-Assay Precision (Precision within an assay) Three samples
  • Intended UseMouse Wif1 ELISA Kit allows for the in vitro quantitative determination of Wif1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: Mouse WIF1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for WIF1 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: G29-W379) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for WIF1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin- Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse WIF1 amount of sample captured in plate. Background/Introduction: Wnt inhibitory factor 1 is a protein that in humans is encoded by the WIF1 gene. The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. Additionally, this protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers.
  • Mouse Wnt inhibitory factor 1 Protein information
  • Uniprot ID WIF1_MOUSE
  • Uniprot AC Q9WUA1;
  • UniGene Mm.32831;
  • GeneID 24117
  • KEGG mmu:24117;
  • Mouse Wnt inhibitory factor 1 Protein SEQUENCE

  • SEQUENCE 379 AA; 41590 MW; E3765F2642B2BC9A CRC64;

    MARRRAFPAF ALRLWSILPC LLLLRADAGQ PPEESLYLWI DAHQARVLIG

    FEEDILIVSE GKMAPFTHDF RKAQQRMPAI PVNIHSMNFT WQAAGQAEYF

    YEFLSLRSLD KGIMADPTVN VPLLGTVPHK ASVVQVGFPC LGKQDGVAAF

    EVNVIVMNSE GNTILRTPQN AIFFKTCQQA ECPGGCRNGG FCNERRVCEC

    PDGFYGPHCE KALCIPRCMN GGLCVTPGFC ICPPGFYGVN CDKANCSTTC

    FNGGTCFYPG KCICPPGLEG EQCELSKCPQ PCRNGGKCIG KSKCKCPKGY

    QGDLCSKPVC EPGCGAHGTC HEPNKCQCRE GWHGRHCNKR YGASLMHAPR

    PAGAGLERHT PSLKKAEDRR DPPESNYIW

  • UCSC uc007hfj.2; mouse.;



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