Human AP3B1 ELISA Kit

Alternative name
Human adaptor protein complex AP-3 subunit beta-1 ELISA Kit;Human adaptor-related protein complex 3 subunit beta-1 ELISA Kit;Human beta-3A-adaptin ELISA Kit;Human clathrin assembly protein complex 3 beta-1 large chain ELISA Kit;Human ADTB3A ELISA Kit;Human ADTB3 ELISA Kit;Human HPS ELISA Kit;Human HPS2 ELISA Kit;Human PE ELISA Kit;Human adaptor related protein complex 3 beta 1 subunit ELISA Kit;Human AP-3 complex subunit beta-1 ELISA Kit;Human AP-3 complex beta-3A subunit ELISA Kit;
Catalog
E000726
species
Human
GeneAP3B1
Standard Curve
Other Species Mouse Ap3b1 ELISA Kit Bovine AP3B1 ELISA Kit Canine AP3B1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of AP3b1. No significant cross-reactivity or interference between AP3b1 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of AP3b1 is typically less than 0.064ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding tw
Intended UseHuman AP3B1 ELISA Kit allows for the in vitro quantitative determination of AP3B1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to AP3b1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to AP3b1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain AP3b1, biotin-conjugated antibody and enzyme- conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of AP3b1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human AP-3 complex subunit beta-1 Protein information
Uniprot ID AP3B1_HUMAN
Uniprot AC O00203; E5RJ68; O00580; Q7Z393; Q9HD66;
UniGene Hs.532091;
GeneID 8546
KEGG hsa:8546;
Human AP-3 complex subunit beta-1 Protein SEQUENCE
SEQUENCE 1094 AA; 121320 MW; AC683CE18EF90555 CRC64;
MSSNSFPYNE QSGGGEATEL GQEATSTISP SGAFGLFSSD LKKNEDLKQM
LESNKDSAKL DAMKRIVGMI AKGKNASELF PAVVKNVASK NIEIKKLVYV
YLVRYAEEQQ DLALLSISTF QRALKDPNQL IRASALRVLS SIRVPIIVPI
MMLAIKEASA DLSPYVRKNA AHAIQKLYSL DPEQKEMLIE VIEKLLKDKS
TLVAGSVVMA FEEVCPDRID LIHKNYRKLC NLLVDVEEWG QVVIIHMLTR
YARTQFVSPW KEGDELEDNG KNFYESDDDQ KEKTDKKKKP YTMDPDHRLL
IRNTKPLLQS RNAAVVMAVA QLYWHISPKS EAGIISKSLV RLLRSNREVQ
YIVLQNIATM SIQRKGMFEP YLKSFYVRST DPTMIKTLKL EILTNLANEA
NISTLLREFQ TYVKSQDKQF AAATIQTIGR CATNILEVTD TCLNGLVCLL
SNRDEIVVAE SVVVIKKLLQ MQPAQHGEII KHMAKLLDSI TVPVARASIL
WLIGENCERV PKIAPDVLRK MAKSFTSEDD LVKLQILNLG AKLYLTNSKQ
TKLLTQYILN LGKYDQNYDI RDRTRFIRQL IVPNVKSGAL SKYAKKIFLA
QKPAPLLESP FKDRDHFQLG TLSHTLNIKA TGYLELSNWP EVAPDPSVRN
VEVIELAKEW TPAGKAKQEN SAKKFYSESE EEEDSSDSSS DSESESGSES
GEQGESGEEG DSNEDSSEDS SSEQDSESGR ESGLENKRTA KRNSKAKGKS
DSEDGEKENE KSKTSDSSND ESSSIEDSSS DSESESEPES ESESRRVTKE
KEKKTKQDRT PLTKDVSLLD LDDFNPVSTP VALPTPALSP SLMADLEGLH
LSTSSSVISV STPAFVPTKT HVLLHRMSGK GLAAHYFFPR QPCIFGDKMV
SIQITLNNTT DRKIENIHIG EKKLPIGMKM HVFNPIDSLE PEGSITVSMG
IDFCDSTQTA SFQLCTKDDC FNVNIQPPVG ELLLPVAMSE KDFKKEQGVL
TGMNETSAVI IAAPQNFTPS VIFQKVVNVA NVGAVPSGQD NIHRFAAKTV
HSGSLMLVTV ELKEGSTAQL IINTEKTVIG SVLLRELKPV LSQG
UCSC uc003kfj.5; human. [O00203-1];

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