Mouse Vdr ELISA Kit

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  • Alternative name

    Mouse VDR ELISA Kit;Mouse 1,25-dihydroxyvitamin D3 receptor ELISA Kit;Mouse nuclear receptor subfamily 1 group I member 1 ELISA Kit;Mouse Nr1i1 ELISA Kit;Mouse vitamin D receptor ELISA Kit;Mouse vitamin D3 receptor ELISA Kit;

  • Catalog
    E072554
  • species
    Mouse
  • GeneVdr
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Vitamin D Receptor (VDR). No significant cross-reactivity or interference between Vitamin D Receptor (VDR) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.134ng/mL.
  • Intended UseMouse Vdr ELISA Kit allows for the in vitro quantitative determination of Vdr , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vitamin D Receptor (VDR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vitamin D Receptor (VDR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vitamin D Receptor (VDR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vitamin D Receptor (VDR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse Vitamin D3 receptor Protein information
  • Uniprot ID VDR_MOUSE
  • Uniprot AC P48281; Q922X0;
  • UniGene Mm.245084;
  • GeneID 22337
  • KEGG mmu:22337;
  • Mouse Vitamin D3 receptor Protein SEQUENCE
  • SEQUENCE 422 AA; 47834 MW; 4614DD4A129F2732 CRC64;

    MEAMAASTSL PDPGDFDRNV PRICGVCGDR ATGFHFNAMT CEGCKGFFRR

    SMKRKALFTC PFNGDCRITK DNRRHCQACR LKRCVDIGMM KEFILTDEEV

    QRKREMIMKR KEEEALKDSL RPKLSEEQQH IIAILLDAHH KTYDPTYADF

    RDFRPPIRAD VSTGSYSPRP TLSFSGDSSS NSDLYTPSLD MMEPASFSTM

    DLNEEGSDDP SVTLDLSPLS MLPHLADLVS YSIQKVIGFA KMIPGFRDLT

    SDDQIVLLKS SAIEVIMLRS NQSFTLDDMS WDCGSQDYKY DITDVSRAGH

    TLELIEPLIK FQVGLKKLNL HEEEHVLLMA ICIVSPDRPG VQDAKLVEAI

    QDRLSNTLQT YIRCRHPPPG SHQLYAKMIQ KLADLRSLNE EHSKQYRSLS

    FQPENSMKLT PLVLEVFGNE IS

  • UCSC uc007xlk.1; mouse.;



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