Human AP2M1 ELISA Kit

Alternative name
Human AP-2 mu chain ELISA Kit;Human adaptin-mu2 ELISA Kit;Human adaptor protein complex AP-2 subunit mu ELISA Kit;Human adaptor-related protein complex 2 subunit mu ELISA Kit;Human clathrin assembly protein complex 2 mu medium chain ELISA Kit;Human clathrin coat assembly protein AP50 ELISA Kit;Human clathrin coat-associated protein AP50 ELISA Kit;Human HA2 50 kDA subunit ELISA Kit;Human plasma membrane adaptor AP-2 50 kDa protein ELISA Kit;Human CLAPM1 ELISA Kit;Human KIAA0109 ELISA Kit;Human AP50 ELISA Kit;Human mu2 ELISA Kit;Human adaptor related protein complex 2 mu 1 subunit ELISA Kit;Human AP-2 complex subunit mu ELISA Kit;Human AP-2 mu 2 chain ELISA Kit;Human clathrin adaptor complex AP2, mu subunit ELISA Kit;Human clathrin assembly protein complex 2 medium chain ELISA Kit;Human clathrin coat adaptor protein AP50 ELISA Kit;Human clathrin-associated/assembly/adaptor protein, medium 1 ELISA Kit;Human plasma membrane adaptor AP-2 50kDA protein ELISA Kit;
Catalog
E000725
species
Human
GeneAP2M1
Standard Curve
Other Species Mouse Ap2m1 ELISA Kit Bovine AP2M1 ELISA Kit Monkey AP2M1 ELISA Kit Rat Ap2m1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Adaptor Related Protein Complex 2 Mu 1 (AP2m1). No significant cross-reactivity or interference between Adaptor Related Protein Complex 2 Mu 1 (AP2m1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.057ng/mL.
Intended UseHuman AP2M1 ELISA Kit allows for the in vitro quantitative determination of AP2M1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adaptor Related Protein Complex 2 Mu 1 (AP2m1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adaptor Related Protein Complex 2 Mu 1 (AP2m1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adaptor Related Protein Complex 2 Mu 1 (AP2m1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adaptor Related Protein Complex 2 Mu 1 (AP2m1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human AP-2 complex subunit mu Protein information
Uniprot ID AP2M1_HUMAN
Uniprot AC Q96CW1; A6NE12; D3DNT1; P20172; P53679;
UniGene Hs.518460;
GeneID 1173
KEGG hsa:1173;
Human AP-2 complex subunit mu Protein SEQUENCE
SEQUENCE 435 AA; 49655 MW; 82803219BA279954 CRC64;
MIGGLFIYNH KGEVLISRVY RDDIGRNAVD AFRVNVIHAR QQVRSPVTNI
ARTSFFHVKR SNIWLAAVTK QNVNAAMVFE FLYKMCDVMA AYFGKISEEN
IKNNFVLIYE LLDEILDFGY PQNSETGALK TFITQQGIKS QHQTKEEQSQ
ITSQVTGQIG WRREGIKYRR NELFLDVLES VNLLMSPQGQ VLSAHVSGRV
VMKSYLSGMP ECKFGMNDKI VIEKQGKGTA DETSKSGKQS IAIDDCTFHQ
CVRLSKFDSE RSISFIPPDG EFELMRYRTT KDIILPFRVI PLVREVGRTK
LEVKVVIKSN FKPSLLAQKI EVRIPTPLNT SGVQVICMKG KAKYKASENA
IVWKIKRMAG MKESQISAEI ELLPTNDKKK WARPPISMNF EVPFAPSGLK
VRYLKVFEPK LNYSDHDVIK WVRYIGRSGI YETRC
UCSC uc003fmw.4; human. [Q96CW1-1];

Category

Human AP2M1 ELISA Kit