Mouse Vldlr ELISA Kit

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  • Alternative name

    Mouse VLDL receptor ELISA Kit;Mouse VLDL-R ELISA Kit;Mouse AA408956 ELISA Kit;Mouse AI451093 ELISA Kit;Mouse AW047288 ELISA Kit;Mouse very low density lipoprotein receptor ELISA Kit;Mouse very low-density lipoprotein receptor ELISA Kit;

  • Catalog
    E072464
  • species
    Mouse
  • GeneVldlr
  • Standard CurveMouse Vldlr ELISA Kit
  • Other Species Human VLDLR ELISA KitChicken VLDLR ELISA KitRabbit VLDLR ELISA KitRat Vldlr ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of VLDLR. No significant cross-reactivity or interference between VLDLR and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between VLDLR and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse Vldlr ELISA Kit allows for the in vitro quantitative determination of Vldlr , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCardiovascular
  • Product Description
    specifical
    Principle of the assay: VLDLR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-VLDLR antibody and an VLDLR-HRP conjugate. The assay sample and buffer are incubated together with VLDLR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the VLDLR concentration since VLDLR from samples and VLDLR-HRP conjugate compete for the anti-VLDLR antibody binding site. Since the number of sites is limited, as more sites are occupied by VLDLR from the sample, fewer sites are left to bind VLDLR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VLDLR concentration in each sample is interpolated from this standard curve.
  • Mouse Very low-density lipoprotein receptor Protein information
  • Uniprot ID VLDLR_MOUSE
  • Uniprot AC P98156; Q64022;
  • UniGene Mm.4141;
  • GeneID 22359
  • KEGG mmu:22359;
  • Mouse Very low-density lipoprotein receptor Protein SEQUENCE
  • SEQUENCE 873 AA; 96373 MW; 08F09F93825195CB CRC64;

    MGTSARWALW LLLALCWAPR DSGATASGKK AKCDSSQFQC TNGRCITLLW

    KCDGDEDCAD GSDEKNCVKK TCAESDFVCK NGQCVPNRWQ CDGDPDCEDG

    SDESPEQCHM RTCRINEISC GARSTQCIPV SWRCDGENDC DNGEDEENCG

    NITCSADEFT CSSGRCVSRN FVCNGQDDCD DGSDELDCAP PTCGAHEFQC

    STSSCIPLSW VCDDDADCSD QSDESLEQCG RQPVIHTKCP TSEIQCGSGE

    CIHKKWRCDG DPDCKDGSDE VNCPSRTCRP DQFECEDGSC IHGSRQCNGI

    RDCVDGSDEV NCKNVNQCLG PGKFKCRSGE CIDMSKVCDQ EQDCRDWSDE

    PLKECHINEC LVNNGGCSHI CKDLVIGYEC DCAAGFELID RKTCGDIDEC

    QNPGICSQIC INLKGGYKCE CSRGYQMDLA TGVCKAVGKE PSLIFTNRRD

    IRKIGLERKE YIQLVEQLRN TVALDADIAA QKLFWADLSQ KAIFSASIDD

    KVGRHFKMID NVYNPAAIAV DWVYKTIYWT DAASKTISVA TLDGAKRKFL

    FNSDLREPAS IAVDPLSGFV YWSDWGEPAK IEKAGMNGFD RRPLVTEDIQ

    WPNGITLDLV KSRLYWLDSK LHMLSSVDLN GQDRRIVLKS LEFLAHPLAL

    TIFEDRVYWI DGENEAVYGA NKFTGSELAT LVNNLNDAQD IIVYHELVQP

    SGKNWCEDDM ENGGCEYLCL PAPQINDHSP KYTCSCPNGY NLEENGRECQ

    STSTPVTYSE TKDINTTDIL RTSGLVPGGI NVTTAVSEVS VPPKGTSAAW

    AILPLLLLVM AAVGGYLMWR NWQHKNMKSM NFDNPVYLKT TEEDLSIDIG

    RHSASVGHTY PAISVVSTDD DLA

  • UCSC uc008hbt.2; mouse.;


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