Mouse VEGF ELISA Kit

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  • Alternative name

    Vascular endothelial growth factor A ELISA KIT; Vascular permeability factor ELISA KIT; VPFVEGFA ELISA KIT; VEGF ELISA KIT; VEGF-A ELISA KIT; VPF ELISA KIT

  • Catalog
    E072436
  • species
    Mouse
  • GeneVEGF
  • Standard CurveMouse VEGF ELISA Kit
  • Other Species Human EG-VEGF ELISA KitHuman EG VEGF ELISA KitMouse EG-VEGF ELISA KitMouse VEGF-165B ELISA KitMouse VEGF.C ELISA KitMouse VEGF.D ELISA KitMouse VEGF-E ELISA KitMouse VEGF-A ELISA KitMouse VEGF-A ELISA ELISA KitMouse VEGF-B ELISA KitMouse VEGF-C ELISA KitMouse VEGF-D ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseMouse VEGF ELISA Kit allows for the in vitro quantitative determination of VEGF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyCytokines, Growth Factors & Hormones; Elisa Kits; Metabolism Assays; Other Metabolism-Related Assays; Metabolism Assays; Metabolism Assays (A-Z)
  • Product Description
    specifical
    Product Information: VEGF (mouse) ELISA Kit: Colorimetric Assay for Quantitative measurement of murine VEGF in serum, plasma, urine & cell culture supernatants. Detection Range: 15.6 pg/ml - 1000 pg/ml. 100 assays. Background: Vascular endothelial growth factor (VEGF) is a signaling messenger involved in endothelial cell proliferation and angiogenesis. Biobool's mouse VEGF ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A rat monoclonal antibody specific for mouse VEGF is coated on a 96-well plate. Standards and test samples are added to the wells and VEGF present in a sample is bound by the immobilized antibody. A biotinylated polyclonal antibody from goat specific for VEGF is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the mouse VEGF captured onto the plate. This kit recognizes both the 164 and 120 amino acid residue forms of mouse VEGF. This ELISA kit shows no detectable cross-reactivity with related proteins. Detection Range: 15.6 pg/ml - 1000 pg/ml. Sensitivity: < 2 pg/ml. Summary: Detection method- Absorbance (450 nm) Species reactivity-human Application- Detection of IgG Ab to TPO



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