Mouse Hras ELISA Kit

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  • Alternative name

    Mouse H-Ras-1 ELISA Kit;Mouse transforming protein P21 ELISA Kit;Mouse c-H-ras ELISA Kit;Mouse p21ras ELISA Kit;Mouse Hras1 ELISA Kit;Mouse H-ras ELISA Kit;Mouse Ha-ras ELISA Kit;Mouse Harvey-ras ELISA Kit;Mouse Hras-1 ELISA Kit;Mouse Kras2 ELISA Kit;Mouse c-Ha-ras ELISA Kit;Mouse c-rasHa ELISA Kit;Mouse ras ELISA Kit;Mouse Harvey rat sarcoma virus oncogene ELISA Kit;Mouse GTPase HRas ELISA Kit;Mouse H-ras 1 protein ELISA Kit;Mouse Harvey rat sarcoma virus oncogene 1 ELISA Kit;Mouse c-Ha-ras p21 protein ELISA Kit;Mouse c-Ha-ras transgene ELISA Kit;

  • Catalog
  • species
  • GeneHras
  • Standard CurveMouse Hras ELISA Kit
  • Other Species Human HRAS ELISA KitChicken HRAS ELISA KitRat Hras ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseMouse Hras ELISA Kit allows for the in vitro quantitative determination of Hras , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCancer
  • Product Description
    Intended Uses: This HRAS ELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofHRAS in the sample, thisHRAS ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusHRAS concentration. The concentration ofHRAS in the samples is then determined by comparing the O.D. of the samples to the standard curve Principle of the Assay: This HRAS enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific forHRAS. Standards or samples are then added to the microtiter plate wells andHRAS if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount ofHRAS present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific forHRAS are added to each well to "sandwich" theHRAS immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that containHRAS and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
  • Mouse GTPase HRas, N-terminally processed Protein information
  • Uniprot ID RASH_MOUSE
  • Uniprot AC Q61411; F7BIB2; Q6P716; Q80WD2; Q811B9;
  • UniGene Mm.334313;
  • GeneID 15461
  • KEGG mmu:15461;
  • Mouse GTPase HRas, N-terminally processed Protein SEQUENCE
  • SEQUENCE 189 AA; 21298 MW; EE6DC2D933E2856A CRC64;





  • UCSC uc009kjv.2; mouse. [Q61411-2];

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