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Alternative name
Human Beta-chemokine exodus-3 ELISA Kit;Human CK beta-11 ELISA Kit;Human epstein-Barr virus-induced molecule 1 ligand chemokine ELISA Kit;Human EBI1 ligand chemokine ELISA Kit;Human ELC ELISA Kit;Human Macrophage inflammatory protein 3 beta ELISA Kit;Human MIP-3-beta ELISA Kit;Human small-inducible cytokine A19 ELISA Kit;Human MIP3B ELISA Kit;Human SCYA19 ELISA Kit;Human CKb11 ELISA Kit;Human MIP-3b ELISA Kit;Human C-C motif chemokine ligand 19 ELISA Kit;Human C-C motif chemokine 19 ELISA Kit;Human CC chemokine ligand 19 ELISA Kit;Human EBI1-ligand chemokine ELISA Kit;Human beta chemokine exodus-3 ELISA Kit;Human chemokine (C-C motif) ligand 19 ELISA Kit;Human exodus-3 ELISA Kit;Human macrophage inflammatory protein 3-beta ELISA Kit;Human small inducible cytokine subfamily A (Cys-Cys), member 19 ELISA Kit;
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Catalog
E007200
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species
Human
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GeneCCL19
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SpecificityNatural and recombinant human CCL19
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity Preparation and Storage
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Intended UseHuman CCL19 ELISA Kit allows for the in vitro quantitative determination of CCL19 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole ELISA kit at 4℃
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Product Description
specificalPrinciple of the assay: human CCL19 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL19 has been precoated onto 96-well plates. Standards(E.coli, G22-S98) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL19 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL19 amount of sample captured in plate.
Background: Chemokine (C-C motif) ligand 19 (CCL19) is a small cytokine belonging to the CC chemokine family that is also known as EBI1 ligand chemokine (ELC) and macrophage inflammatory protein-3-beta (MIP-3-beta). Using PCR of human-rodent hybrids, the CCL19 gene was map to human chromosome 9p13, and the CCL19 gene is not part of the CC chemokine gene cluster on chromosome 17. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs.
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Human C-C motif chemokine 19 Protein information
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Uniprot ID
CCL19_HUMAN
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Uniprot AC
Q99731; O00697; O00736;
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UniGene
Hs.50002;
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GeneID
6363
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KEGG
hsa:6363;
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Human C-C motif chemokine 19 Protein SEQUENCE
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SEQUENCE 98 AA; 10993 MW; 0D2516DB2EB1C81B CRC64;
MALLLALSLL VLWTSPAPTL SGTNDAEDCC LSVTQKPIPG YIVRNFHYLL
IKDGCRVPAV
VFTTLRGRQL CAPPDQPWVE RIIQRLQRTS AKMKRRSS
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UCSC
uc003zvn.4; human.;
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