Mouse ULBP1 ELISA Kit

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  • Alternative name

    NKG2D ligand 1 ELISA KIT; ALCAN-beta ELISA KIT; Retinoic acid early transcript 1I ELISA KIT; UL16-binding protein 1ULBP1 ELISA KIT; N2DL1 ELISA KIT; RAET1I ELISA KIT; N2DL-1 ELISA KIT; NKG2DL1 ELISA KIT

  • Catalog
    E071994
  • species
    Mouse
  • GeneULBP1
  • Standard CurveMouse ULBP1 ELISA Kit
  • Other Species Human ULBP1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ULBP1. No significant cross-reactivity or interference between ULBP1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ULBP1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse ULBP1 ELISA Kit allows for the in vitro quantitative determination of ULBP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyImmunology
  • Product Description
    specifical
    Principle of the assay: ULBP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ULBP1 antibody and an ULBP1-HRP conjugate. The assay sample and buffer are incubated together with ULBP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ULBP1 concentration since ULBP1 from samples and ULBP1-HRP conjugate compete for the anti-ULBP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ULBP1 from the sample, fewer sites are left to bind ULBP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ULBP1 concentration in each sample is interpolated from this standard curve.


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