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  • Alternative name

    UDP-glucuronosyltransferase 1-6 ELISA KIT; Phenol-metabolizing UDP-glucuronosyltransferase ELISA KIT; UDP-glucuronosyltransferase 1-F ELISA KIT; UGT-1F ELISA KIT; UGT1F ELISA KIT; UDP-glucuronosyltransferase 1A6UGT1A6 ELISA KIT; GNT1 ELISA KIT; UGT1 ELISA KIT; UDPGT 1-6 ELISA KIT; UGT1*6 ELISA KIT; UGT1-06 ELISA KIT; UGT1.6 ELISA KIT; UGT-1F ELISA KIT; UGT1F ELISA KIT

  • Catalog
  • species
  • GeneUDPGT
  • Standard CurveMouse UDPGT ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of UGT. No significant cross-reactivity or interference between UGT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between UGT and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseMouse UDPGT ELISA Kit allows for the in vitro quantitative determination of UDPGT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Intended Uses: This UGT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse UGT. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||UGT ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-UGT antibody and an UGT-HRP conjugate. The assay sample and buffer are incubated together with UGT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the UGT concentration since UGT from samples and UGT-HRP conjugate compete for the anti-UGT antibody binding site. Since the number of sites is limited, as more sites are occupied by UGT from the sample, fewer sites are left to bind UGT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The UGT concentration in each sample is interpolated from this standard curve.

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