Mouse UCP-1 ELISA Kit

Contact Us
  • Alternative name

    Tryptophan rich basic protein ELISA KIT; Tryptophan rich basic proteinWRB ELISA KIT;

  • Catalog
    E071936
  • species
    Mouse
  • GeneUCP-1
  • SpecificityThis kit recognizes natural and recombinant Mouse UCP-1. No significant cross-reactivity or interference between Mouse UCP-1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of Mouse UCP-1 is 0.094ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).
  • Intended UseMouse UCP-1 ELISA Kit allows for the in vitro quantitative determination of UCP-1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to UCP-1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for UCP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain UCP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of UCP-1. You can calculate the concentration of UCP-1 in the samples by comparing the OD of the samples to the standard curve.



Inquiry About Mouse UCP-1 ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!