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Alternative name
Human chemokine CC-2 ELISA Kit;Human HCC-2 ELISA Kit;Human Leukotactin-1 ELISA Kit;Human LKN-1 ELISA Kit;Human MIP-1 delta ELISA Kit;Human macrophage inflammatory protein 5 ELISA Kit;Human MIP-5 ELISA Kit;Human MRP-2B ELISA Kit;Human NCC-3 ELISA Kit;Human small-inducible cytokine A15 ELISA Kit;Human MIP5 ELISA Kit;Human NCC3 ELISA Kit;Human SCYA15 ELISA Kit;Human HMRP-2B ELISA Kit;Human LKN1 ELISA Kit;Human MIP-1D ELISA Kit;Human SCYL3 ELISA Kit;Human SY15 ELISA Kit;Human C-C motif chemokine ligand 15 ELISA Kit;Human C-C motif chemokine 15 ELISA Kit;Human chemokine (C-C motif) ligand 15 ELISA Kit;Human leukotactin 1 ELISA Kit;Human new CC chemokine 3 ELISA Kit;Human small inducible cytokine subfamily A (Cys-Cys), member 15 ELISA Kit;
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Catalog
E007190
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species
Human
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GeneCCL15
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SpecificityNatural and recombinant human CCL15
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity< 10pg/ml
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Intended UseHuman CCL15 ELISA Kit allows for the in vitro quantitative determination of CCL15 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole ELISA kit at 4℃
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Product Description
specificalPrinciple of the assay: human CCL15 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL15 has been precoated onto 96-well plates. Standards(E.coli, Q22-I113) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL15 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL15 amount of sample captured in plate.
Background: Chemokine (C-C motif) ligand 15 (CCL15) is a small cytokine belonging to the CC chemokine family that is also known as leukotactin-1, MIP5 and HCC-2. CCL15 is expressed in liver, small intestine, colon, and in certain leukocytes and macrophages of the lung. It is chemotactic for neutrophils, monocytes, and lymphocytes and elicits its effects by binding to cell surface chemokine receptors like CCR1 and CCR3. The human CCL15 gene spans four exons and is located in a head-to-tail orientation on chromosome 17 with the gene of another CC chemokine known as CCL14.
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Human CCL15(29-92) Protein information
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Uniprot ID
CCL15_HUMAN
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Uniprot AC
Q16663; B2RU34; E1P651; Q9UM74;
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UniGene
Hs.272493;
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GeneID
6359
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KEGG
hsa:6359;
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Human CCL15(29-92) Protein SEQUENCE
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SEQUENCE 113 AA; 12248 MW; 0BA0FCE7B8A30A04 CRC64;
MKVSVAALSC LMLVAVLGSQ AQFINDAETE LMMSKLPLEN PVVLNSFHFA
ADCCTSYISQ
SIPCSLMKSY FETSSECSKP GVIFLTKKGR QVCAKPSGPG
VQDCMKKLKP YSI
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UCSC
uc032gbw.1; human.;
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