Human CCL14 ELISA Kit

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  • Alternative name

    Human chemokine CC-1/CC-3 ELISA Kit;Human HCC-1/HCC-3 ELISA Kit;Human HCC-1(1-74) ELISA Kit;Human NCC-2 ELISA Kit;Human small-inducible cytokine A14 ELISA Kit;Human NCC2 ELISA Kit;Human SCYA14 ELISA Kit;Human CC-1 ELISA Kit;Human CC-3 ELISA Kit;Human CKB1 ELISA Kit;Human HCC-1 ELISA Kit;Human HCC-3 ELISA Kit;Human MCIF ELISA Kit;Human SCYL2 ELISA Kit;Human SY14 ELISA Kit;Human C-C motif chemokine ligand 14 ELISA Kit;Human C-C motif chemokine 14 ELISA Kit;Human chemokine (C-C motif) ligand 14 ELISA Kit;Human chemokine CC-3 ELISA Kit;Human hemofiltrate CC chemokine 1 ELISA Kit;Human new CC chemokine 2 ELISA Kit;Human small inducible cytokine subfamily A (Cys-Cys), member 14 ELISA Kit;

  • Catalog
    E007189
  • species
    Human
  • GeneCCL14
  • Other Species Mouse CCL14 ELISA Kit
  • SpecificityNatural and recombinant human CCL14
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 10pg/ml
  • Intended UseHuman CCL14 ELISA Kit allows for the in vitro quantitative determination of CCL14 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: human CCL14 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL14 has been precoated onto 96-well plates. Standards(E.coli, T20-N93) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL14 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL14 amount of sample captured in plate. Background: Chemokine (C-C motif) ligand 14 (CCL14) is a small cytokine belonging to the CC chemokine family. It is also commonly known as HCC-1. It is produced as a protein precursor that is processed to generate a mature active protein containing 74 amino acids that and is 46% identical in amino acid composition to CCL3 and CCL4. This chemokine is expressed in various tissues including spleen, bone marrow, liver, muscle, and gut. CCL14 activates monocytes, but does not induce their chemotaxis. In addition, HCC-1 enhanced the proliferation of CD34+ myeloid progenitor cells. It was as effective as MIP-1 alpha, but about 100-fold less potent. Human CCL14 is located on chromosome 17 within a cluster of other chemokines belonging to the CC family.
  • Human HCC-1(9-74) Protein information
  • Uniprot ID CCL14_HUMAN
  • Uniprot AC Q16627; E1P649; E1P650; Q13954;
  • UniGene Hs.272493; Hs.740501;
  • GeneID 6358
  • KEGG hsa:6358;
  • Human HCC-1(9-74) Protein SEQUENCE
  • SEQUENCE 93 AA; 10678 MW; DDDB899DC9148836 CRC64;

    MKISVAAIPF FLLITIALGT KTESSSRGPY HPSECCFTYT TYKIPRQRIM

    DYYETNSQCS KPGIVFITKR GHSVCTNPSD KWVQDYIKDM KEN

  • UCSC uc010wcq.2; human. [Q16627-1];


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