Human CEBPB ELISA Kit

Alternative name
Human C/EBP beta ELISA Kit;Human Liver activator protein ELISA Kit;Human LAP ELISA Kit;Human Liver-enriched inhibitory protein ELISA Kit;Human LIP ELISA Kit;Human nuclear factor NF-IL6 ELISA Kit;Human transcription factor 5 ELISA Kit;Human TCF-5 ELISA Kit;Human TCF5 ELISA Kit;Human C/EBP-beta ELISA Kit;Human IL6DBP ELISA Kit;Human NF-IL6 ELISA Kit;Human CCAAT/enhancer binding protein beta ELISA Kit;Human CCAAT/enhancer-binding protein beta ELISA Kit;Human CCAAT/enhancer binding protein (C/EBP), beta ELISA Kit;Human interleukin 6-dependent DNA-binding protein ELISA Kit;Human nuclear factor of interleukin 6 ELISA Kit;Human transcription factor C/EBP beta ELISA Kit;
Catalog
E007164
species
Human
GeneCEBPB
Standard Curve
Other Species Mouse Cebpb ELISA Kit Bovine CEBPB ELISA Kit Chicken CEBPB ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of CCAAT/Enhancer Binding Protein Beta (CEBPb). No significant cross-reactivity or interference between CCAAT/Enhancer Binding Protein Beta (CEBPb) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.061ng/mL.
Intended UseHuman CEBPB ELISA Kit allows for the in vitro quantitative determination of CEBPB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CCAAT/Enhancer Binding Protein Beta (CEBPb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to CCAAT/Enhancer Binding Protein Beta (CEBPb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CCAAT/Enhancer Binding Protein Beta (CEBPb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CCAAT/Enhancer Binding Protein Beta (CEBPb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human CCAAT/enhancer-binding protein beta Protein information
Uniprot ID CEBPB_HUMAN
Uniprot AC P17676; A8K671; Q96IH2; Q9H4Z5;
UniGene Hs.517106; Hs.719041; Hs.720603;
GeneID 1051
KEGG hsa:1051;
Human CCAAT/enhancer-binding protein beta Protein SEQUENCE
SEQUENCE 345 AA; 36106 MW; 9B725BD6CCAA771D CRC64;
MQRLVAWDPA CLPLPPPPPA FKSMEVANFY YEADCLAAAY GGKAAPAAPP
AARPGPRPPA GELGSIGDHE RAIDFSPYLE PLGAPQAPAP ATATDTFEAA
PPAPAPAPAS SGQHHDFLSD LFSDDYGGKN CKKPAEYGYV SLGRLGAAKG
ALHPGCFAPL HPPPPPPPPP AELKAEPGFE PADCKRKEEA GAPGGGAGMA
AGFPYALRAY LGYQAVPSGS SGSLSTSSSS SPPGTPSPAD AKAPPTACYA
GAAPAPSQVK SKAKKTVDKH SDEYKIRRER NNIAVRKSRD KAKMRNLETQ
HKVLELTAEN ERLQKKVEQL SRELSTLRNL FKQLPEPLLA SSGHC
UCSC uc002xvi.4; human. [P17676-1];

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