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Alternative name
Tumor necrosis factor ligand superfamily member 11 ELISA KIT; Osteoclast differentiation factor ELISA KIT; ODFTNFSF11 ELISA KIT; OPGL ELISA KIT; RANKL ELISA KIT; TRANCE ELISA KIT; ODF ELISA KIT; OPGL ELISA KIT; RANKL ELISA KIT; TRANCE ELISA KIT
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Catalog
E071517
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species
Mouse
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GeneTNFSF11/RANKL
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity< 9.4pg/ml
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Intended UseMouse TNFSF11/RANKL ELISA Kit allows for the in vitro quantitative determination of TNFSF11/RANKL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse TNFSF11 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse TNFSF11 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse TNFSF11 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofMouse TNFSF11 can be calculated.
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