Intended UseMouse TNF-alpha ELISA Kit allows for the in vitro quantitative determination of TNF-alpha , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with TNF-alpha. Standards and samples are pipetted into the wells with a biotin-conjugated TNF-alpha and avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNF-alpha antibody bound in the initial step. The color development is stopped and the intensity of the color is measured.
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