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Human CCR4 ELISA Kit

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  • Alternative name

    Human C-C CKR-4 ELISA Kit;Human CC-CKR-4 ELISA Kit;Human CCR-4 ELISA Kit;Human CCR4 ELISA Kit;Human K5-5 ELISA Kit;Human CMKBR4 ELISA Kit;Human CD194 ELISA Kit;Human CKR4 ELISA Kit;Human ChemR13 ELISA Kit;Human HGCN:14099 ELISA Kit;Human C-C motif chemokine receptor 4 ELISA Kit;Human C-C chemokine receptor type 4 ELISA Kit;Human chemokine (C-C motif) receptor 4 ELISA Kit;Human chemokine (C-C) receptor 4 ELISA Kit;
  • Catalog
    E007142
  • species
    Human
  • GeneCCR4
  • Standard CurveHuman CCR4 ELISA Kit
  • Other Species Mouse Ccr4 ELISA KitCanine CCR4 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman CCR4 ELISA Kit allows for the in vitro quantitative determination of CCR4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyImmunology
  • Product Description
    specifical    Principle of the Assay: CDK6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CDK6 antibody and an CDK6-HRP conjugate. The assay sample and buffer are incubated together with CDK6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CDK6 concentration since CDK6 from samples and CDK6-HRP conjugate compete for the anti-CDK6 antibody binding site. Since the number of sites is limited, as more sites are occupied by CDK6 from the sample, fewer sites are left to bind CDK6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CDK6 concentration in each sample is interpolated from this standard curve.
  • Human C-C chemokine receptor type 4 Protein information
  • Uniprot ID CCR4_HUMAN
  • Uniprot AC P51679; Q9ULY6; Q9ULY7;
  • UniGene Hs.184926;
  • GeneID 1233
  • KEGG hsa:1233;
  • Human C-C chemokine receptor type 4 Protein SEQUENCE

  • SEQUENCE 360 AA; 41403 MW; 51EBE12AD1FAFABF CRC64;

    MNPTDIADTT LDESIYSNYY LYESIPKPCT KEGIKAFGEL FLPPLYSLVF

    VFGLLGNSVV VLVLFKYKRL RSMTDVYLLN LAISDLLFVF SLPFWGYYAA

    DQWVFGLGLC KMISWMYLVG FYSGIFFVML MSIDRYLAIV HAVFSLRART

    LTYGVITSLA TWSVAVFASL PGFLFSTCYT ERNHTYCKTK YSLNSTTWKV

    LSSLEINILG LVIPLGIMLF CYSMIIRTLQ HCKNEKKNKA VKMIFAVVVL

    FLGFWTPYNI VLFLETLVEL EVLQDCTFER YLDYAIQATE TLAFVHCCLN

    PIIYFFLGEK FRKYILQLFK TCRGLFVLCQ YCGLLQIYSA DTPSSSYTQS

    TMDHDLHDAL

  • UCSC uc003cfg.2; human.;


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